Pérez-Gallardo Lucía, Gómez Marta, Parra Pilar, Sánchez Juana, Palou Andreu, Serra Francisca
Department of Biochemistry, Molecular Biology and Physiology, University of Valladolid, Campus de Soria, Soria, Spain.
Br J Nutr. 2009 May;101(10):1463-6. doi: 10.1017/S0007114508102446. Epub 2008 Nov 6.
The aim of this work was to assess the effects of a high-fat diet enriched in Ca, which accompanies lower body fat deposition, on mineral depots, as well as to assess the potential role of adaptive thermogenesis in mice. Male mice were fed ad libitum a high-fat (43 %) diet with a Ca content of 4 g/kg from calcium carbonate (control group) or 12 g/kg (42 % from milk powder and the rest from calcium carbonate) (Ca group) for 56 d. Body weight, food intake and urine were periodically collected. Tissue samples were collected when the mice were killed and the composition was determined. Expression of uncoupling proteins was determined by Western blotting. Mineral content was measured by flame atomic absorption spectrometry. Lower body weight gain and fat accretion was found in the Ca group. This could not be attributable to lower gross energy intake or to activation of adaptive thermogenesis. Although significant urine mineral loss was found in the Ca group, preservation of mineral depots in bone was observed. Our data support the fact that adding more Ca to the diet, using a combination of calcium carbonate plus milk powder containing among other things higher Zn and Mg, contributes to counteracting obesity and improving lipid metabolism.
本研究旨在评估富含钙的高脂饮食（伴随较低的体脂沉积）对矿物质储备的影响，以及评估适应性产热在小鼠中的潜在作用。雄性小鼠自由采食高脂（43%）饮食，碳酸钙组钙含量为4 g/kg（对照组），钙组钙含量为12 g/kg（42%来自奶粉，其余来自碳酸钙），持续56天。定期收集体重、食物摄入量和尿液。处死小鼠时收集组织样本并测定其成分。通过蛋白质免疫印迹法测定解偶联蛋白的表达。通过火焰原子吸收光谱法测量矿物质含量。钙组小鼠体重增加和脂肪堆积较少。这并非归因于总能量摄入降低或适应性产热的激活。尽管钙组出现显著的尿矿物质流失，但观察到骨骼中的矿物质储备得以保留。我们的数据支持以下事实：在饮食中添加更多的钙，使用碳酸钙与含有较高锌和镁等成分的奶粉组合，有助于对抗肥胖并改善脂质代谢。
