Gerdes H H, Rudolf R
Department of Neurobiology, University of Heidelberg, Federal Republic of Germany.
Protoplasma. 1999;209(1-2):1-8. doi: 10.1007/BF01415695.
Since the advent of green-fluorescent protein (GFP) technology there has been an explosion of interest in applying this molecule to cell biology. This review summarizes new insights in secretory membrane traffic obtained by the use of GFP fusion proteins. Transport steps between the endoplasmic reticulum and the Golgi apparatus, intra-Golgi traffic, and transport from the Golgi to the plasma membrane are discussed. In addition, insights into the dynamics of the Golgi compartment in plant cells and in mitotic mammalian cells have been included. We conclude that membrane traffic in the secretory pathway appears to be much more dynamic and diverse than previously thought and that GFP promises to be a powerful means to unravel these complex processes.
自绿色荧光蛋白（GFP）技术问世以来，将该分子应用于细胞生物学的兴趣激增。本综述总结了通过使用GFP融合蛋白在分泌膜运输方面获得的新见解。讨论了内质网与高尔基体之间的运输步骤、高尔基体内部运输以及从高尔基体到质膜的运输。此外，还纳入了对植物细胞和有丝分裂哺乳动物细胞中高尔基体区室动态的见解。我们得出结论，分泌途径中的膜运输似乎比以前认为的更加动态和多样，并且GFP有望成为揭示这些复杂过程的有力手段。
