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铜绿假单胞菌中的蛋白质分泌：xcpA基因编码一种与肺炎克雷伯菌分泌功能蛋白PulO同源的整合内膜蛋白。

Protein secretion in Pseudomonas aeruginosa: the xcpA gene encodes an integral inner membrane protein homologous to Klebsiella pneumoniae secretion function protein PulO.

作者信息

Bally M, Ball G, Badere A, Lazdunski A

机构信息

Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.

出版信息

J Bacteriol. 1991 Jan;173(2):479-86. doi: 10.1128/jb.173.2.479-486.1991.

DOI:10.1128/jb.173.2.479-486.1991
PMID:1898929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207036/
Abstract

xcp mutations have pleiotropic effects on the secretion of proteins in Pseudomonas aeruginosa PAO. The nucleotide sequence of a 1.2-kb DNA fragment that complements the xcp-1 mutation has been determined. Sequence analysis shows the xcpA gene product to be a 31.8-kDa polypeptide, with a highly hydrophobic character. This is consistent with a localization in the cytoplasmic membrane in P. aeruginosa, determined after specific expression of the xcpA gene under control of the T7 phi 10 promoter. A very strong homology was found between XcpA and PulO, a membrane protein required for pullulanase secretion in Klebsiella pneumoniae. This suggests the existence of a signal sequence-dependent secretion process common to these two unrelated gram-negative bacteria.

摘要

xcp突变对铜绿假单胞菌PAO中蛋白质的分泌具有多效性影响。已确定了一个可互补xcp - 1突变的1.2 kb DNA片段的核苷酸序列。序列分析表明，xcpA基因产物是一种31.8 kDa的多肽，具有高度的疏水性。这与在T7 phi 10启动子控制下xcpA基因特异性表达后确定的铜绿假单胞菌细胞质膜定位一致。在XcpA和PulO之间发现了很强的同源性，PulO是肺炎克雷伯菌中支链淀粉酶分泌所需的一种膜蛋白。这表明这两种不相关的革兰氏阴性细菌存在一种依赖信号序列的共同分泌过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6127/207036/29fc86da037e/jbacter00092-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6127/207036/a65a3ddf1bdd/jbacter00092-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6127/207036/29fc86da037e/jbacter00092-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6127/207036/a65a3ddf1bdd/jbacter00092-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6127/207036/29fc86da037e/jbacter00092-0078-a.jpg

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