Kanyicska B, Burris T P, Freeman M E
Department of Biological Science, Florida State University, Tallahassee 32306.
Biochem Biophys Res Commun. 1991 Jan 15;174(1):338-43. doi: 10.1016/0006-291x(91)90525-c.
The influence of endothelin-3 (ET-3) on anterior pituitary hormone secretion was investigated over a wide range of concentrations (from 10(-14) to 10(-6) M) and incubation times (from 4 to 48 hours). ET-3 elicited a concentration-dependent inhibition of prolactin (PRL) secretion and stimulated the release of luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH) from primary monolayer cultures of anterior pituitary cells derived from female rats. The responsiveness of different pituitary cells to ET-3 differs markedly in terms of onset and duration: the maximal inhibition of PRL secretion occurred after 12 hours and the stimulation of LH, FSH and TSH reached the maximum after 4, 48 and 48 hours of incubation, respectively. These data corroborate the concept that ET-3 has an important role as a neuroendocrine modulator. Moreover, the data presented suggest different intracellular mechanisms underlying ET-3 actions.
研究了内皮素-3（ET-3）在广泛浓度范围（从10^(-14)至10^(-6) M）和孵育时间（从4至48小时）对垂体前叶激素分泌的影响。ET-3引起催乳素（PRL）分泌的浓度依赖性抑制，并刺激来自雌性大鼠垂体前叶细胞原代单层培养物中促黄体生成素（LH）、促卵泡激素（FSH）和促甲状腺激素（TSH）的释放。不同垂体细胞对ET-3的反应性在起效时间和持续时间方面存在显著差异：PRL分泌的最大抑制在12小时后出现，而LH、FSH和TSH的刺激分别在孵育4、48和48小时后达到最大值。这些数据证实了ET-3作为神经内分泌调节剂具有重要作用这一概念。此外，所呈现的数据表明ET-3作用背后存在不同的细胞内机制。
