Soto F R M, Viana W G, Mucciolo G C B, Hosomi F Y M, Vannucchi C I, Mazzei C P, Eyherabide A R, de Fátima Lúcio C, Dias R A, de Azevedo S S
Center for Sanitary Surveillance and Zoonoses Control Tereza Rodrigues de Camargo, SP, CEP 18150-000, Brazil.
Reprod Domest Anim. 2009 Dec;44(6):927-31. doi: 10.1111/j.1439-0531.2008.01119.x.
The aim of this research was to evaluate the efficacy of zinc gluconate associated with dimethyl sulphoxide (DMSO) for chemical neutering in canine males. Fifteen sexually mature male dogs were divided in two groups, named control and treated. An injection was administered to both testicles, at a concentration of 26.2 mg zinc gluconate per ml and 0.5% DMSO in the treated group (11 dogs). The control group was given injections of saline solution (four dogs). Clinical examination and blood collection for a haemogram were done both before and after drug injection. There were 12 spermograms performed to analyse sperm motility, sperm vigour, ejaculate volume, testicle size, pathology and sperm concentrations. Libido was also measured. An ultrasound examination and histopathology were performed at the end of the experiment. Dogs' libido after chemical injection was reduced by over 50%. The spermogram analysis showed final mean results of 14.54% for sperm motility, 0.72 of sperm vigour and 37,150 per million spermatozoa per millilitre, values considered below the necessary levels at which fertilization can occur. Ultrasound and histopathology analyses of testicles for the treated group revealed more intense injuries when compared with the control group, with compromised testicular parenchyma and a decrease of germ cell number leading to total atrophy, indicating that the treatment reduced the fertilizing potential of male dogs, promoting a possible subfertile status.
本研究的目的是评估葡萄糖酸锌与二甲基亚砜（DMSO）联合用于雄性犬化学去势的疗效。15只性成熟雄性犬被分为两组，即对照组和治疗组。对治疗组（11只犬）的双侧睾丸进行注射，注射浓度为每毫升含26.2毫克葡萄糖酸锌和0.5% DMSO。对照组注射生理盐水（4只犬）。在药物注射前后均进行临床检查并采集血液做血常规检查。共进行了12次精液分析，以分析精子活力、精子活率、射精量、睾丸大小、病理情况和精子浓度。还测量了性欲。实验结束时进行了超声检查和组织病理学检查。化学注射后犬的性欲降低了50%以上。精液分析最终平均结果显示，精子活力为14.54%，精子活率为0.72，每毫升精液中精子数量为37150个/百万，这些数值被认为低于受精所需的必要水平。与对照组相比，治疗组睾丸的超声和组织病理学分析显示损伤更严重，睾丸实质受损，生殖细胞数量减少导致完全萎缩，这表明该治疗降低了雄性犬的受精潜力，可能导致亚生育状态。
