Rafatmah Dorna, Mogheiseh Asghar, Eshghi Davoud
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O.Box 71441-69155, Shiraz, Fars Iran.
Basic Clin Androl. 2019 Aug 1;29:12. doi: 10.1186/s12610-019-0092-8. eCollection 2019.
Chemical sterilization with zinc gluconate is being developed due to its permanent contraceptive effect in prepubertal dogs. In this study, five healthy adult dogs were selected randomly. Semen samples were collected and analyzed before the study to confirm normal testicular function. Under general anesthesia, pH neutralized zinc gluconate was injected directly into their testes. Testes diameter, ultrasonographic appearance, changes in the percentage of white blood cells, and testosterone concentration were monitored twice a week before and 1 month after the injection. At the end of the study, the dogs were castrated and their testes were removed for histopathological evaluation.
The general health of all dogs was normal after the injection. The appearance of testicular swelling was limited within 2 days of treatment. The average diameter of left and right testes was 2.48 and 2.03 cm before the injection and reached to diameter 2.12 and 2.15 cm, respectively, at the end of the study. Serum testosterone concentration was 4.2 ng/ml at the start and 4 ng/ml at the end of the study. The percentage of white blood cells at the start and end of the study were within normal ranges reported for dogs. Histopathological analyses indicate a degeneration of germ cells in seminiferous tubules, but Leydig cells retained their structure.
Therefore, It is inferred that the injection of pH neutralized zinc gluconate into the adult dogs' testes resulted in the loss of sperm-producing tissue without affecting the production of testosterone and the general health of adult dogs.
由于葡萄糖酸锌在青春期前犬类中具有永久避孕作用，正在研发用其进行化学绝育。在本研究中，随机选择了五只健康成年犬。在研究前采集精液样本并进行分析，以确认睾丸功能正常。在全身麻醉下，将pH中和的葡萄糖酸锌直接注入它们的睾丸。在注射前和注射后1个月，每周监测两次睾丸直径、超声图像、白细胞百分比变化和睾酮浓度。在研究结束时，对犬进行去势并切除其睾丸进行组织病理学评估。
注射后所有犬的总体健康状况正常。睾丸肿胀的外观在治疗后2天内得到限制。注射前左右睾丸的平均直径分别为2.48厘米和2.03厘米，在研究结束时分别达到2.12厘米和2.15厘米。研究开始时血清睾酮浓度为4.2纳克/毫升，研究结束时为4纳克/毫升。研究开始和结束时白细胞百分比在犬类报告的正常范围内。组织病理学分析表明生精小管中的生殖细胞发生退化，但间质细胞保留了其结构。
因此，推断向成年犬睾丸注射pH中和的葡萄糖酸锌会导致产生精子的组织丧失，而不会影响成年犬的睾酮分泌和总体健康。
