Sheridan P J, Koka S, Ewoldsen N O, Lefebvre C A, Lavin M T
Department of Otolaryngology-Head and Neck Surgery, University of Nebraska Medical Center, Omaha 68583-0750, USA.
Int J Prosthodont. 1997 Jan-Feb;10(1):73-7.
This in vitro study examined the effect of eluate from heat-activated, chemically activated, and microwave-activated denture base resins on cell viability of primary cultures of human gingival fibroblasts. Eluates corresponding to 24, 48, 72, and 96 hours of resin disk immersion were prepared. Fibroblasts were plated at a density of 3 x 10(4) cells in 96-well plates and exposed to a medium containing eluate. After 24 hours, the cytotoxic effect was determined by cellular mitochondrial function. The effect of eluates was compared to control cultures containing culture medium without eluate. Results indicated that at all time periods tested, all three resins leached materials that were cytotoxic to the fibroblasts. Eluate from chemically activated resin disks was more cytotoxic than eluate from heat-activated and microwave-activated disks. In general, cytotoxicity appeared to diminish as disk immersion time was increased. The greatest cytotoxic effect on cell viability was observed with eluates recovered after 24 hours of disk immersion, and the least cytotoxic effect was observed with eluates recovered after 96 hours of immersion.
这项体外研究考察了热固化、化学固化和微波固化义齿基托树脂洗脱液对人牙龈成纤维细胞原代培养细胞活力的影响。制备了树脂盘浸泡24、48、72和96小时后的洗脱液。将成纤维细胞以3×10⁴个细胞的密度接种于96孔板中，并暴露于含有洗脱液的培养基中。24小时后，通过细胞线粒体功能测定细胞毒性作用。将洗脱液的作用与含有不含洗脱液的培养基的对照培养物进行比较。结果表明，在所有测试时间段，所有三种树脂均释放出对成纤维细胞具有细胞毒性的物质。化学固化树脂盘的洗脱液比热固化和微波固化树脂盘的洗脱液细胞毒性更强。总体而言，随着盘浸泡时间的增加，细胞毒性似乎减弱。在盘浸泡24小时后回收的洗脱液对细胞活力的细胞毒性作用最大，而在浸泡96小时后回收的洗脱液细胞毒性作用最小。
