Resident, Department of Restorative Dentistry, Indiana University School of Dentistry, Indiana University, Indianapolis, Ind; Implant fellow, Department of Restorative Dentistry and Biomaterials Sciences, Harvard School of Dental Medicine, Boston, Mass; Department of Substitutive Dental Sciences, College of Dentistry, Taibah University, Al Madinah Al Munawwarah, KSA.
J Prosthet Dent. 2013 Oct;110(4):296-302. doi: 10.1016/S0022-3913(13)60379-0.
Interim acrylic resins release agents that alter cytokine expression in the surrounding tissues, which could alter extracellular matrix degradation.
The purpose of the study was to evaluate the responses of human epidermal keratinocytes to eluates of interim acrylic resins in regards to cytokine expression and cell-mediated collagen degradation.
Specimens of 4 different interim acrylic resins (HI-I, Jet Acrylic, SNAP acrylic, and Protemp Plus) were placed in Epilife medium for 48 hours and the eluates collected. The cells were incubated for 72 hours in nontoxic concentrations of the eluates. Cytotoxicity was evaluated with lactate dehydrogenase assays and cytokine expression with cytokine antibody arrays. Collagen degradation was determined with a collagen type I assay. The experiments were performed 3 times. Data were analyzed with 1-way and mixed-model ANOVA (α=.05).
None of the eluates were cytotoxic. Cytokine expression from the heat-activated polymethyl methacrylate resin group was significantly less for interleukin-3, but significantly greater for interlukin-7. Expression for the chemically activated polymethyl methacrylate resin group was significantly less for growth-regulated oncogene-α, interleukin-1α, and interleukin-3. Expression for the chemically activated polyethyl methacrylate resin group was significantly less for interleukin-1α and interleukin-3, but significantly greater for interleukin-13 and monocytes chemoattractant protein-3. The cytokine expression induced by chemically activated bis-acryl composite resin was significantly greater for granulocyte-macrophage colony stimulating factor, interleukin-7, and monocytes chemoattractant protein-3, but significantly less for growth-regulated oncogene-α. Collagen degradation was not significantly different in any of the groups.
The eluates used were not cytotoxic and did not induce cell-mediated collagen degradation. Some significant changes in cytokine expression were noted.
临时丙烯酸树脂释放剂会改变周围组织中的细胞因子表达，从而改变细胞外基质的降解。
本研究旨在评估人表皮角质细胞对临时丙烯酸树脂浸提液的反应，观察细胞因子表达和细胞介导的胶原蛋白降解情况。
将 4 种不同的临时丙烯酸树脂（HI-I、Jet Acrylic、SNAP acrylic 和 Protemp Plus）标本置于 Epilife 培养基中 48 小时，收集浸提液。将细胞在无毒浓度的浸提液中孵育 72 小时。通过乳酸脱氢酶测定法评估细胞毒性，通过细胞因子抗体阵列评估细胞因子表达。通过 I 型胶原蛋白测定法确定胶原蛋白降解情况。实验重复 3 次。采用单向和混合模型方差分析（α=.05）进行数据分析。
没有一种浸提液具有细胞毒性。热激活聚甲基丙烯酸甲酯树脂组的白细胞介素-3 表达显著减少，但白细胞介素-7 表达显著增加。化学激活聚甲基丙烯酸甲酯树脂组的生长调节癌基因-α、白细胞介素-1α 和白细胞介素-3 的表达显著减少。化学激活聚乙基丙烯酸甲酯树脂组的白细胞介素-1α 和白细胞介素-3 的表达显著减少，但白细胞介素-13 和单核细胞趋化蛋白-3 的表达显著增加。化学激活双丙烯酸复合树脂诱导的细胞因子表达中，粒细胞-巨噬细胞集落刺激因子、白细胞介素-7 和单核细胞趋化蛋白-3 的表达显著增加，而生长调节癌基因-α 的表达显著减少。在任何一组中，胶原蛋白降解均无显著差异。
所用浸提液无细胞毒性，不诱导细胞介导的胶原蛋白降解。观察到细胞因子表达的一些显著变化。
