Martinez-Martinez L, Pascual A, Perea E J
Department of Microbiology, School of Medicine, University of Sevilla, Spain.
J Med Microbiol. 1991 Jan;34(1):7-12. doi: 10.1099/00222615-34-1-7.
The adherence of six non-mucoid and three mucoid strains of Pseudomonas aeruginosa to polyvinyl chloride (PVC), polyurethane (PU) and siliconised latex (SL) was evaluated by a radiometric method and scanning electronmicroscopy. Initially greater numbers of mucoid than non-mucoid strains adhered to all three materials. Hydrophobic non-mucoid strains adhered more efficiently than hydrophilic strains. Numbers of adherent non-mucoid P. aeruginosa cells increased with time, reaching a peak, which was different for each strain, at 1-4 h for PU, 4 h-2 days for SL and 2-3 days for PVC; thereafter a gradual decrease was observed, maximal and final values of adherence being higher with PVC and SL than with PU. Adherence of mucoid strains increased with time in 3-5 days, until a steady state was reached. We conclude that although non-mucoid strains of P. aeruginosa initially colonise plastic surfaces better than mucoid strains, mucoid strains also persist on these surfaces.
采用放射性测定法和扫描电子显微镜对6株非黏液型和3株黏液型铜绿假单胞菌在聚氯乙烯（PVC）、聚氨酯（PU）和硅化乳胶（SL）上的黏附情况进行了评估。最初，黏液型菌株比非黏液型菌株在这三种材料上的黏附数量更多。疏水性非黏液型菌株比亲水性菌株黏附更有效。非黏液型铜绿假单胞菌的黏附细胞数量随时间增加，达到峰值，每种菌株的峰值不同，PU在1 - 4小时达到峰值，SL在4小时至2天达到峰值，PVC在2 - 3天达到峰值；此后观察到逐渐下降，PVC和SL的最大和最终黏附值高于PU。黏液型菌株的黏附在3 - 5天内随时间增加，直至达到稳定状态。我们得出结论，虽然铜绿假单胞菌的非黏液型菌株最初在塑料表面的定植比黏液型菌株更好，但黏液型菌株也能在这些表面持续存在。
