Martínez-Martínez L, Pascual A, Perea E J
Department of Microbiology, School of Medicine, University of Sevilla, Spain.
J Hosp Infect. 1990 Nov;16(4):311-8. doi: 10.1016/0195-6701(90)90003-7.
The effect of polyvinylchloride (PVC), polyurethane (PU) and siliconized latex (SL) catheters on the survival and growth of six non-mucoid and three mucoid strains of Pseudomonas aeruginosa was evaluated. Pseudomonas aeruginosa (1 x 10(8)) was incubated in PBS alone (control) or with 30 1-cm length segments of each catheter and the number of viable microorganisms was determined after 8 h, 1, 2, 5, 7 and 10 days. The presence of PVC catheters significantly favoured the survival and growth of non-mucoid strains in comparison to the control (P less than 0.05 at 5 days, P less than 0.01 at 7 days and thereafter); a similar result was observed with SL catheters (P less than 0.05 at 2 days, P less than 0.01 at 5 days and thereafter). No differences were observed with PU catheters. The number of mucoid microorganisms decreased with time in all controls and suspensions containing segments of catheter, but non-mucoid revertants appeared and quickly increased in the presence of PVC and SL (but not PU) catheters. Eluates of PBS previously containing PVC or SL segments induced a 100- to 500-fold increase in the growth of a non-mucoid strain in comparison with PBS alone. It is concluded that some plastic catheters can release substance(s) that favour the viability of P. aeruginosa.
评估了聚氯乙烯（PVC）导管、聚氨酯（PU）导管和硅化乳胶（SL）导管对六种非黏液型和三种黏液型铜绿假单胞菌菌株存活和生长的影响。将铜绿假单胞菌（1×10⁸）分别单独在PBS（对照）中培养，或与每种导管的30段1厘米长的片段一起培养，并在8小时、1天、2天、5天、7天和10天后测定活菌数量。与对照相比，PVC导管的存在显著促进了非黏液型菌株的存活和生长（5天时P＜0.05，7天及之后P＜0.01）；SL导管也观察到类似结果（2天时P＜0.05，5天及之后P＜0.01）。PU导管未观察到差异。在所有对照和含有导管片段的悬液中，黏液型微生物数量随时间减少，但在存在PVC和SL（而非PU）导管的情况下，出现了非黏液型回复突变体并迅速增加。先前含有PVC或SL片段的PBS洗脱液与单独的PBS相比，可使非黏液型菌株的生长增加100至500倍。得出的结论是，一些塑料导管可释放有利于铜绿假单胞菌存活的物质。
