Buch J P, Lipshultz L I, Smith R G
Scott Department of Urology, Baylor College of Medicine, Houston, Texas.
Fertil Steril. 1991 Feb;55(2):358-62. doi: 10.1016/s0015-0282(16)54130-2.
It is generally assumed that the immunological activity of the follicle-stimulating hormone (FSH) molecule, as reported in the radioimmunoassay (RIA), is identical to the molecule's biological activity. To test the validity of this assumption, the receptor-binding and immunological activities of FSH in the serum of 35 infertile males and 11 fertile males were determined by radioreceptor assay (RRA) and by the standard RIA. The results were analyzed in terms of the ratio of binding (RRA) to immunological (RIA) activity x 100%, referred to as the B/I%, for each patient based on data from the RRA and RIA, respectively. The B/I% for fertile men ranged from 44% to 113% (mean of 80%). In the group of 11 infertile men with normal FSH levels by RIA (50 to 300 ng/mL), there was no significant difference in B/I% (range of 27% to 99%, mean of 59%) from the fertile controls. However, a statistically significant decrease in B/I% (range of 7% to 35%, mean of 18%) was noted in the 24 hypergonadotropic infertile men with RIA levels of serum FSH greater than 300 ng/mL. These significant discrepancies between receptor-binding and immunological activities of serum FSH raise the question of whether RIAs alone are valid parameters for the endocrine evaluation of infertile men with elevated serum FSH levels.
一般认为，放射免疫测定法（RIA）中所报告的促卵泡激素（FSH）分子的免疫活性与其生物活性相同。为检验这一假设的正确性，采用放射受体分析法（RRA）和标准RIA对35名不育男性和11名生育力正常男性血清中的FSH受体结合活性和免疫活性进行了测定。根据每位患者分别来自RRA和RIA的数据，以结合（RRA）与免疫（RIA）活性之比×100%（称为B/I%）来分析结果。生育力正常男性的B/I%范围为44%至113%（平均为80%）。在11名RIA检测FSH水平正常（50至300 ng/mL）的不育男性组中，其B/I%（范围为27%至99%，平均为59%）与生育力正常的对照组相比无显著差异。然而，在24名血清FSH的RIA水平大于300 ng/mL的高促性腺激素性不育男性中，观察到B/I%有统计学意义的降低（范围为7%至35%，平均为18%）。血清FSH的受体结合活性与免疫活性之间的这些显著差异，引发了对于仅用RIA作为血清FSH水平升高的不育男性内分泌评估的有效参数是否合适的质疑。
