Moodley Arshnee, Stegger Marc, Ben Zakour Nouri L, Fitzgerald J Ross, Guardabassi Luca
Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 4, Frederiksberg C, 1870, Denmark.
Vet Microbiol. 2009 Mar 30;135(3-4):320-6. doi: 10.1016/j.vetmic.2008.09.070. Epub 2008 Sep 30.
A putative staphylococcal protein A (spa) gene was discovered in the genome of Staphylococcus pseudintermedius and used for developing a species-specific spa typing protocol. Thirty-one clinical methicillin-resistant S. pseudintermedius (MRSP) isolates from dogs and cats in four countries were characterized by spa typing, pulsed-field gel electrophoresis (PFGE) and staphylococcal cassette chromosome (SCCmec) typing. The results indicated the occurrence of two MRSP clones that acquired distinct SCCmec elements in Europe (t02, PFGE type A, SCCmec type III,) and California (t06, PFGE type B, SCCmec type V). Sequence analysis of mecA revealed the occurrence of four alleles (mecA1 to mecA4), which correlated with the geographical origin of the isolates and enabled discrimination of two distinct subtypes within the European clone. The newly developed spa typing method appeared to be a promising tool for easy and rapid typing of MRSP, either alone or in combination with SCCmec and mecA typing for fine-structure epidemiological analysis.
在中间型葡萄球菌基因组中发现了一个假定的葡萄球菌蛋白A（spa）基因，并用于开发一种物种特异性的spa分型方案。采用spa分型、脉冲场凝胶电泳（PFGE）和葡萄球菌盒式染色体（SCCmec）分型对来自四个国家的犬猫临床耐甲氧西林中间型葡萄球菌（MRSP）分离株31株进行了鉴定。结果表明，在欧洲（t02，PFGE A型，SCCmec III型）和加利福尼亚（t06，PFGE B型，SCCmec V型）出现了两个获得不同SCCmec元件的MRSP克隆。mecA序列分析显示存在四个等位基因（mecA1至mecA4），这与分离株的地理来源相关，并能够区分欧洲克隆中的两个不同亚型。新开发的spa分型方法似乎是一种很有前景的工具，可单独或与SCCmec和mecA分型结合用于MRSP的简便快速分型，以进行精细结构的流行病学分析。
