Frank Linda A, Kania Stephen A, Kirzeder Elizabeth M, Eberlein Laura C, Bemis David A
Department of Small Animal Clinical Sciences, University of Tennessee, Knoxville, TN 37996-4544, USA.
Vet Dermatol. 2009 Oct;20(5-6):496-501. doi: 10.1111/j.1365-3164.2009.00826.x.
To determine the zoonotic risk from meticillin-resistant staphylococcal species or transfer of resistance genes between dogs with pyoderma and their owners, 25 dog-owner pairs were studied. Cultures were obtained from the dog's lesions and the owner's nasal cavity on the initial visit. Staphylococcus isolates were identified and antimicrobial susceptibility tests were performed by the Kirby-Bauer disk diffusion method. Presence of the mecA gene was determined by PCR. Staphylococcal Cassette Chromosome (SCCmec) typing was performed by multiplex PCR. Eighteen dogs had a meticillin-resistant staphylococcal species, with meticillin-resistant S. pseudintermedius (MRSP) isolated from 15 dogs. MRSP was isolated from two owners of dogs with MRSP skin infections. Both organisms had the same susceptibility pattern and SCCmec type. MRSP was not isolated from the owners after treating both dogs for 1 month. At least one coagulase-negative Staphylococcus spp. (CoNS) was isolated from each owner, with meticillin resistance found in 16 (64%) of the isolates. The mecA gene was identified in all but two of the meticillin-resistant Staphylococcus spp. Multiplex PCR identified SCCmec type V in all MRSP. The mecA gene-possessing CoNS isolates from owners contained either SCCmec type IVa or IVc. In conclusion, MRSP colonization of owners appeared to be uncommon and transient. Human nasal carriage of meticillin-resistant CoNS was common, but the SCCmec types were different from those in the canine MRSP isolates. Owners do not appear to be at great risk of zoonotic transfer of organisms or antimicrobial resistance genes from dogs with MRSP infections, but the findings should be confirmed with a much larger cohort.
为了确定耐甲氧西林葡萄球菌物种带来的人畜共患病风险，或脓皮病犬与其主人之间耐药基因的转移情况，对25对犬主进行了研究。在初次就诊时，从犬的病变部位和主人的鼻腔获取培养物。对葡萄球菌分离株进行鉴定，并采用 Kirby-Bauer 纸片扩散法进行药敏试验。通过聚合酶链反应（PCR）检测mecA基因的存在情况。采用多重PCR对葡萄球菌盒式染色体（SCCmec）进行分型。18只犬感染了耐甲氧西林葡萄球菌物种，其中15只犬分离出耐甲氧西林中间型葡萄球菌（MRSP）。从患有MRSP皮肤感染的犬的两位主人身上分离出了MRSP。两种菌株具有相同的药敏模式和SCCmec类型。对两只犬进行1个月的治疗后，未从其主人身上分离出MRSP。每位主人至少分离出一种凝固酶阴性葡萄球菌属（CoNS），其中16株（64%）分离株对甲氧西林耐药。除两株耐甲氧西林葡萄球菌物种外，其余所有菌株均鉴定出mecA基因。多重PCR在所有MRSP中均鉴定出SCCmec V型。从主人身上分离出的携带mecA基因的CoNS菌株含有SCCmec IVa型或IVc型。总之，主人感染MRSP似乎并不常见且是短暂的。人类鼻腔携带耐甲氧西林CoNS很常见，但SCCmec类型与犬MRSP分离株中的不同。患有MRSP感染的犬向主人进行人畜共患病原体或抗菌药物耐药基因转移似乎风险不大，但这一发现应通过更大规模的队列研究加以证实。
