Ruscher Claudia, Lübke-Becker Antina, Wleklinski Claus-G, Soba Alexandra, Wieler Lothar H, Walther Birgit
Institute of Microbiology and Epizootics, Free University Berlin, Philippstrasse 13, 10115 Berlin, Germany.
Vet Microbiol. 2009 Apr 14;136(1-2):197-201. doi: 10.1016/j.vetmic.2008.10.023. Epub 2008 Oct 31.
In this study the prevalence of Methicillin-resistant Staphylococcus pseudintermedius (MRSP) in clinical specimens of different animal species was defined by investigating a total of 16,103 clinical samples originating from veterinary facilities of five German federal states in 2007. Of all samples examined, 72 were positive for MRSP, giving an overall prevalence of 0.45%. In clinical specimens originating from small animals the prevalence was 0.58% (n=67; dogs n=61 and cats n=6), while samples from equidaes revealed a prevalence of 0.10% (n=5; horses n=4, donkey n=1). Forty-six representative phenotypically identified MRSP were further differentiated by DNA-based species assignment, PCR detection of mecA, SCCmec-typing and MIC determination. As expected, all 46 isolates were unambiguously proven to be MRSP by sequencing of housekeeping genes pta and cpn60 and being positive for mecA. Furthermore, all isolates harboured the mobile staphylococcal cassette chromosome mec (SCCmec) SCCmecIII. Antibiotic susceptibility testing for 20 different conventional antimicrobial agents disclosed a high rate of multidrug-resistant isolates (45 of 46) displaying an identical or at least similar resistance pattern for non-beta-lactam antimicrobials. The recognized prevalence of MRSP, which have already been shown to be potential zoonotic agents, reflects the recently emerging development of these serious and often multidrug-resistant pathogens in Germany.
在本研究中，通过调查2007年来自德国五个联邦州兽医机构的总共16,103份临床样本，确定了不同动物物种临床样本中耐甲氧西林中间型葡萄球菌（MRSP）的流行情况。在所有检测样本中，72份MRSP呈阳性，总体流行率为0.45%。在源自小型动物的临床样本中，流行率为0.58%（n = 67；狗n = 61，猫n = 6），而马科动物样本的流行率为0.10%（n = 5；马n = 4，驴n = 1）。通过基于DNA的菌种鉴定、mecA的PCR检测、SCCmec分型和最低抑菌浓度测定，对46株表型鉴定的代表性MRSP进行了进一步区分。正如预期的那样，通过管家基因pta和cpn60测序以及mecA呈阳性，所有46株分离株均被明确证明为MRSP。此外，所有分离株都携带移动性葡萄球菌盒式染色体mec（SCCmec）SCCmecIII。对20种不同常规抗菌药物的药敏试验显示，多药耐药分离株的比例很高（46株中有45株），对非β-内酰胺类抗菌药物表现出相同或至少相似的耐药模式。已经证明MRSP是潜在的人畜共患病原体，其公认的流行率反映了德国这些严重且往往具有多重耐药性的病原体最近出现的发展情况。
