Hogg Alison E, Worth Andrew, Beverley Peter, Howard Christopher J, Villarreal-Ramos Bernardo
Institute for Animal Health, Compton, Nr. Newbury, Berkshire RG20 7NN, UK.
Vaccine. 2009 Jan 7;27(2):270-9. doi: 10.1016/j.vaccine.2008.10.053. Epub 2008 Nov 7.
Tuberculosis (TB) remains a worldwide leading cause of death among infectious diseases. Development of safer and more efficacious vaccines requires a basic understanding of the protective mechanisms induced by BCG. Here we show that vaccination of cattle with BCG induces CD8+gamma/deltaTCR-CD45RO+ T-cells that can produce IFN-gamma, up-regulate transcription and expression of perforin, lyse BCG-infected monocyte-derived macrophages (MoMvarphi) and contribute to a reduction in the number of intracellular mycobacteria. We also observed BCG-induced CD8+ responses in vivo. After infection of cattle with Mycobacterium bovis, CD8+gamma/deltaTCR-CD45RO+ cells responded more strongly to M. bovis-infected MoMvarphi than to BCG-infected MoMvarphi. These results indicate that the antigen-specific CD8+ memory response resides in the CD8+gamma/deltaTCR-CD45RO+ cell population.
结核病（TB）仍然是全球传染病致死的主要原因。研发更安全、更有效的疫苗需要对卡介苗（BCG）诱导的保护机制有基本的了解。在此我们表明，用卡介苗给牛接种疫苗可诱导产生CD8+γ/δTCR-CD45RO+ T细胞，这些细胞可产生γ干扰素，上调穿孔素的转录和表达，裂解感染卡介苗的单核细胞衍生巨噬细胞（MoMφ），并有助于减少细胞内分枝杆菌的数量。我们还在体内观察到卡介苗诱导的CD8+反应。在用牛分枝杆菌感染牛后，CD8+γ/δTCR-CD45RO+细胞对感染牛分枝杆菌的MoMφ的反应比对感染卡介苗的MoMφ的反应更强。这些结果表明，抗原特异性CD8+记忆反应存在于CD8+γ/δTCR-CD45RO+细胞群体中。
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