Xiao Ji-dong, Shen Shou-rong
Department of Ultrasound , Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Oct;33(10):937-41.
To examine the expression of NGX6 gene and to investigate its association with the clinico-pathological characteristics in hepatocellular carcinoma(HCC).
Samples from 45 patients were divided into the hepatocellular carcinoma tissue group and the matched paracancerous tissue group. Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of NGX6 gene in the hepatocellular carcinoma and the surrounding normal tissue specimens.
The positive rates of NGX6 in the hepatocellular carcinoma and the matched paracancerous tissues were 35.5%(16/45)and 77.8%(35/45), and the ratios of NGX6/G3PDH mRNA were 0.245+/-0.060 and 0.352+/-0.113.There was significant difference in the 2 groups (P<0.05). The expression of NGX6 gene was related to TNM staging (chi2=6.106,P=0.042)and lymph node metastasis(chi2=5.237,P=0.022)in hepatocellular carcinoma.
There is positive expression of NGX6 in the hepatocellular carcinoma and the matched paracancerous tissues. The low-expression or non-expression of NGX6 gene plays an important role in the gene transcription level in hepatocellular carcinoma.The expression of NGX6 gene is related with TNM staging and lymph node metastasis in hepatocellular carcinoma. Expression of NGX6 might be used as an early indicator of the occurrence and development of hepatocellular carcinoma.
检测NGX6基因在肝细胞癌（HCC）中的表达情况，并探讨其与临床病理特征的关系。
将45例患者的样本分为肝细胞癌组织组和配对癌旁组织组。采用逆转录聚合酶链反应（RT-PCR）检测肝细胞癌及周围正常组织标本中NGX6基因的表达。
NGX6在肝细胞癌组织和配对癌旁组织中的阳性率分别为35.5%（16/45）和77.8%（35/45），NGX6/G3PDH mRNA比值分别为0.245±0.060和0.352±0.113。两组间差异有统计学意义（P<0.05）。NGX6基因的表达与肝细胞癌的TNM分期（χ2=6.106，P=0.042）和淋巴结转移（χ2=5.237，P=0.022）有关。
NGX6在肝细胞癌组织及配对癌旁组织中呈阳性表达。NGX6基因低表达或不表达在肝细胞癌基因转录水平起重要作用。NGX6基因表达与肝细胞癌TNM分期和淋巴结转移有关。NGX6表达可能作为肝细胞癌发生发展的早期指标。
