Suzuki T, Hashimoto Y, Okuno H, Sato H, Nishina H, Iba H
Department of Tumor Virus Research, University of Tokyo.
Jpn J Cancer Res. 1991 Jan;82(1):58-64. doi: 10.1111/j.1349-7006.1991.tb01746.x.
To analyze the transforming activity of c-jun, a Rous sarcoma virus (RSV) variant that carries human c-jun instead of v-src (JH1) was constructed. After infection onto chicken embryo fibroblasts (CEF), JH1 formed foci with a titer comparable to that of wild-type RSV, and the infected cells grew in soft agar, indicating that the human c-jun gene has transforming potential, like the v-jun gene. The expression of Fra-2, one of the recently isolated Fos-related antigens, but not Fos was detected in both JH1-infected CEF and CEF infected with the control retrovirus vector (DS3). Gel shift analysis using nuclear extracts from DS3-infected CEF revealed that the Fra-2/Jun complex contributes to the basal level of AP-1 DNA binding activity. A similar activity was detected in JH1-infected CEF, but these cells have an additional AP-1 binding activity derived from Jun homodimers that seems to play important roles in the cellular transformation.
为了分析c-jun的转化活性，构建了一种携带人c-jun而非v-src的劳斯肉瘤病毒（RSV）变体（JH1）。将JH1感染鸡胚成纤维细胞（CEF）后，JH1形成的集落滴度与野生型RSV相当，且感染细胞能在软琼脂中生长，这表明人c-jun基因具有与v-jun基因类似的转化潜能。在JH1感染的CEF和感染对照逆转录病毒载体（DS3）的CEF中均检测到了Fra-2（一种最近分离出的Fos相关抗原）的表达，但未检测到Fos的表达。使用DS3感染的CEF的核提取物进行凝胶迁移分析表明，Fra-2/Jun复合物有助于AP-1 DNA结合活性的基础水平。在JH1感染的CEF中也检测到了类似的活性，但这些细胞还有一种源自Jun同源二聚体的额外AP-1结合活性，这似乎在细胞转化中发挥重要作用。
