维甲酸增强人-人杂交瘤的抗体产生。
Enhanced antibody production of human-human hybridomas by retinoic acid.
机构信息
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan,
出版信息
Cytotechnology. 2000 Jul;33(1-3):83-8. doi: 10.1023/A:1008155609072.
The enhancement of human monoclonal antibody production by retinoic acid (RA) was evaluated usingthe human-human hybridoma cell line BD9 underserum-free culture condition. The amount of humanIgG secreted by BD9 hybriodmas was enhanced abouteight-fold by treatment with 10(-7) M of RA for 4days. Northern blot analysis showed that both mRNAlevels of the IgG light and heavy chains were markedlyincreased by RA when compared with control without RAtreatment. On the other hand, it was found thatcontinuous treatment of cells with RA was not alwaysrequired to exhibit the enhancing effect, suggestingthat RA may act as a trigger for IgG gene expression. The comparison between extra- and intracellular IgGamounts by immunoblot analysis suggests that thesecretion rate of IgG may be accelerated by RAtreatment. These results suggest that RA may be aneffective culture additive for efficient production ofhuman monoclonal antibody using human-humanhybridomas.
维甲酸（RA）增强人-人杂交瘤细胞系 BD9 在无血清培养条件下产生人单克隆抗体的研究。用 10（-7）M 的 RA 处理 4 天，可使 BD9 杂交瘤细胞分泌的人 IgG 增加约 8 倍。Northern 印迹分析表明，与未经 RA 处理的对照相比，RA 可明显增加 IgG 轻链和重链的 mRNA 水平。另一方面，发现细胞连续用 RA 处理并不总是需要表现出增强效应，这表明 RA 可能作为 IgG 基因表达的触发因子。免疫印迹分析比较细胞内外 IgG 的含量表明，RA 处理可加速 IgG 的分泌速率。这些结果表明，RA 可能是一种有效的培养添加剂，可用于高效生产人-人杂交瘤的人单克隆抗体。
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