Ito Y, Chen G, Imanishi Y
Graduate School of Materials Science, NAIST, Ikoma, Japan.
J Biomater Sci Polym Ed. 1998;9(8):879-90. doi: 10.1163/156856298x00217.
To endow biomaterials with the ability to regulate cell functions such as proliferation, differentiation, and apoptosis, growth factor proteins were covalently immobilized. The proteins were immobilized on various matrices using different chemical methods. It was shown that insulin and epidermal growth factor stimulated cellular functions even after immobilization. Pattern-immobilization of growth factor proteins clearly demonstrated the stimulation by immobilized proteins. In other words, this type of stimulation by non-diffusional growth factors enabled us to regulate tissue formation with artificial biomaterials. The stimulation was enhanced by coimmobilization with adhesion factors. These stimulations due to the immobilized growth factors may mimic juxtacrine stimulation of membrane-anchored growth factors such as heparin-binding epidermal growth factor, transforming growth factor-alpha, and tumor necrosis factor-alpha.
为了赋予生物材料调节细胞功能（如增殖、分化和凋亡）的能力，生长因子蛋白被共价固定。这些蛋白通过不同的化学方法固定在各种基质上。结果表明，胰岛素和表皮生长因子即使在固定后仍能刺激细胞功能。生长因子蛋白的图案化固定清楚地证明了固定化蛋白的刺激作用。换句话说，这种由非扩散性生长因子产生的刺激使我们能够用人工生物材料调节组织形成。通过与黏附因子共固定，刺激作用得到增强。这些由固定化生长因子产生的刺激可能模拟了膜锚定生长因子（如肝素结合表皮生长因子、转化生长因子-α和肿瘤坏死因子-α）的旁分泌刺激。
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