从大籽雪胆中分离得到的葫芦素 E 与肌动蛋白丝的直接相互作用。
Direct interaction of Cucurbitacin E isolated from Alsomitra macrocarpa to actin filament.
机构信息
Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.
出版信息
Cytotechnology. 2008 Jan;56(1):33-9. doi: 10.1007/s10616-007-9100-5. Epub 2007 Nov 7.
Abstract
A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed.
摘要
Alsomitra macrocarpa 叶和枝的甲醇提取物诱导人星状细胞系 (LX-2) 发生明显的细胞形态改变。在其他几种细胞系中也观察到类似的形态改变。从提取物中分离出活性化合物并确定为葫芦素 E（Cuc E）。已知 Cuc E 会导致肌动蛋白细胞骨架明显破坏，这支持了我们的观察结果，但 Cuc E 如何改变肌动蛋白细胞骨架尚未阐明。通过使用聚合和原生及芘标记肌动蛋白的解聚的标准荧光测定法，本研究表明 Cuc E 直接与肌动蛋白相互作用，从而稳定聚合的肌动蛋白。当用 Cuc E 处理外源表达 YFP 标记肌动蛋白的 NIH-3T3 细胞时，首先观察到球状肌动蛋白的聚集，其次观察到包括细胞中纤维状肌动蛋白破坏的肌动蛋白的聚集。
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本文引用的文献
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Cell Mol Life Sci. 2006 Sep;63(18):2119-34. doi: 10.1007/s00018-006-6157-9.
2
Cucurbitacins and cucurbitane glycosides: structures and biological activities.葫芦素和葫芦烷糖苷：结构与生物活性。
Nat Prod Rep. 2005 Jun;22(3):386-99. doi: 10.1039/b418841c. Epub 2005 Apr 4.
3
Immunomodulatory activity of cucurbitacin E isolated from Ecballium elaterium.从喷瓜中分离得到的葫芦素E的免疫调节活性。
Fitoterapia. 2005 Jul;76(5):439-41. doi: 10.1016/j.fitote.2005.02.007.
4
Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.人肝星状细胞系LX - 1和LX - 2：肝纤维化分析的新工具
Gut. 2005 Jan;54(1):142-51. doi: 10.1136/gut.2004.042127.
5
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Chem Biol. 2004 Sep;11(9):1269-77. doi: 10.1016/j.chembiol.2004.07.014.
6
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J Biol Chem. 2000 Feb 18;275(7):5163-70. doi: 10.1074/jbc.275.7.5163.
7
Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization.纽蛋白结合蛋白：一种具有多个SH3结构域的新型纽蛋白结合蛋白可增强肌动蛋白细胞骨架组织。
J Cell Biol. 1999 Jan 11;144(1):59-69. doi: 10.1083/jcb.144.1.59.
8
Formation of F-actin aggregates in cells treated with actin stabilizing drugs.在用肌动蛋白稳定药物处理的细胞中形成F-肌动蛋白聚集体。
Cell Motil Cytoskeleton. 1998;39(2):122-33. doi: 10.1002/(SICI)1097-0169(1998)39:2<122::AID-CM3>3.0.CO;2-8.
9
Jasplakinolide, a novel actin targeting peptide, inhibits cell growth and induces actin filament polymerization in the green alga Micrasterias.茉莉酮酸内酯是一种新型的肌动蛋白靶向肽，它能抑制绿藻微星鼓藻的细胞生长并诱导肌动蛋白丝聚合。
Cell Motil Cytoskeleton. 1997;38(4):365-72. doi: 10.1002/(SICI)1097-0169(1997)38:4<365::AID-CM6>3.0.CO;2-2.
10
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Biochem Biophys Res Commun. 1997 Sep 29;238(3):910-5. doi: 10.1006/bbrc.1997.7407.
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