新型振荡生物反应器高密度培养昆虫细胞和生产重组杆状病毒。
High-density cultivation of insect cells and production of recombinant baculovirus using a novel oscillating bioreactor.
机构信息
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, 300, ROC(e-mail,
出版信息
Cytotechnology. 2003 Sep;42(3):145-53. doi: 10.1023/B:CYTO.0000015841.98225.27.
Abstract
A novel two-compartment bioreactor, BelloCell((R)), was used to cultivate insect cells and a maximum yield of 4.6 x 10(9) cells was attained. The cells were immobilized in a packed bed fixed in the upper chamber, and the bellow in the lower chamber was compressed and released in an alternating fashion. The motion resulted in gentle, cyclic movement of the medium that was contained in the lower chamber and consequently exposed the cells to air in an oscillatory manner, thus rendering adequate aeration and uniform cell distribution in the bed. The baculovirus yield produced in BelloCell((R)) could amount up to 3.3 x 10(17) pfu using as little as 1.1 l medium in the production run. Besides, BelloCell((R)) was extremely easy to handle and operate. These benefits underline the potential of BelloCell((R)) for simple, economical and high-density cell culture and protein/virus production.
摘要
一种新型的双室生物反应器 BelloCell((R)) 被用于培养昆虫细胞，最高可获得 4.6×10(9) 个细胞。细胞被固定在填充床中，位于上部腔室，而下部腔室中的波纹管以交替的方式被压缩和释放。这种运动导致下部腔室中包含的培养基温和、循环移动，从而使细胞以振荡的方式暴露于空气中，从而在床中提供充足的通气和均匀的细胞分布。使用 BelloCell((R)) 生产的杆状病毒产量在生产过程中仅使用 1.1 升培养基就可高达 3.3×10(17)pfu。此外，BelloCell((R)) 非常易于操作和处理。这些优点突显了 BelloCell((R)) 在简单、经济和高密度细胞培养和蛋白质/病毒生产方面的潜力。
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