Department of Molecular Biology, Massachusetts General Hospital, 50 Blossom Street, Boston, MA, 02114, USA.
Cytotechnology. 2000 Mar;32(3):265-7. doi: 10.1023/A:1008127406783.
Manipulation of cells in open dishes is often incompatible withpreservation of sterility. A dish covered with an elastic orstretchable latex or plastic film allows manipulation of cellsthrough the film with preservation of sterility of the cultureand the integrity of the film. The latter forms a ;microglove'for the instrument tip. The idea of manipulation through a thintransparent film is also applicable to general surgery, so thatthe surgeon's hand operates through a film and without a glove.
在开放式培养皿中操作细胞通常与保持无菌状态不兼容。覆盖有弹性或可拉伸乳胶或塑料膜的培养皿允许通过薄膜操作细胞,同时保持培养物的无菌状态和薄膜的完整性。后者为器械尖端形成一个“微型手套”。通过薄而透明的薄膜进行操作的想法也适用于普通外科手术,因此外科医生的手可以通过薄膜操作而无需戴手套。