Bakker D, Van Blitterswijk C A, Daems W T, Grote J J
Ear Nose & Throat Department, University Hospital, Leiden, The Netherlands.
J Biomed Mater Res. 1988 May;22(5):423-39. doi: 10.1002/jbm.820220506.
The biocompatibility of two silicone rubbers, Silastic and Dow Corning Elastomer, and of a polyether and a polyester urethane, a polyether polyester copolymer, and polypropylene oxide was assessed in vitro. These elastomers were selected for assessment as a possible alloplastic tympanic membrane. For these studies use was made of rat middle ear mucosa explants and serially cultured epithelium. The quantitative results were based on epithelial growth curves, the morphological picture was based on the findings in epithelium, and the aging of a biomaterial was simulated. Epithelium morphology was investigated by scanning and transmission electron microscopy and x-ray microanalysis. Quantitative results showed that on Dow Corning Elastomer and polypropylene oxide, cell proliferation was significantly lower compared to normal growth curves. The morphological findings were negative for polypropylene oxide, and did not discriminate between the other biomaterials under study. The simulation results indicated better biocompatibility for the polyurethanes and the polyether polyester copolymer compared with that of polypropylene oxide and both silicone rubbers. Under the simulation conditions, cells exposed to Silastic showed silicon-containing inclusions. These in vitro results suggest that the biocompatibility of the polyurethanes and the polyether polyester copolymer is better than that of both silicone rubbers and polypropylene oxide.
在体外评估了两种硅橡胶（Silastic和道康宁弹性体）、一种聚醚、一种聚酯聚氨酯、一种聚醚聚酯共聚物以及聚环氧丙烷的生物相容性。选择这些弹性体作为可能的人工鼓膜进行评估。在这些研究中，使用了大鼠中耳黏膜外植体和连续培养的上皮细胞。定量结果基于上皮细胞生长曲线，形态学图像基于上皮细胞的观察结果，并模拟了生物材料的老化过程。通过扫描电子显微镜、透射电子显微镜和X射线微分析研究上皮细胞形态。定量结果表明，与正常生长曲线相比，道康宁弹性体和聚环氧丙烷上的细胞增殖明显较低。聚环氧丙烷的形态学观察结果为阴性，在所研究的其他生物材料之间未发现差异。模拟结果表明，与聚环氧丙烷和两种硅橡胶相比，聚氨酯和聚醚聚酯共聚物具有更好的生物相容性。在模拟条件下，暴露于Silastic的细胞显示出含硅包涵体。这些体外结果表明，聚氨酯和聚醚聚酯共聚物的生物相容性优于两种硅橡胶和聚环氧丙烷。
