Department of Veterinary Research and Biotechnology, Razi Vaccine and Serum Research Institute, Ahmadabad Ave, Mashhad, Iran,
Cytotechnology. 2007 May;54(1):49-56. doi: 10.1007/s10616-007-9067-2. Epub 2007 Apr 11.
The water extracts of propolis (WEP) could inhibit growth of different cell lines namely McCoy, HeLa, SP2/0, HEp-2, and BHK21 and stimulate growth of normal cell named human lymphocyte, rat kidney, rat liver, and rat spleen. In these experiments 1 and 2 mg of WEP were added to 1 ml RPMI media with 5% FCS. Cell counts and cell viability of propolis-treated and propolis-free cells were assessed by Trypan blue dye exclusion test and MTT assay. The results showed that in case of McCoy, HeLa, SP20, HEp-2, and BHK21 cell lines, the water extracts of propolis could inhibit cell growth as well as reduction on size of the cells. In contrast the same amount of WEP could stimulate growth of normal cells up to 60% with the same concentration used for cell lines. Thus our study indicates that although WEP consists only of the soluble part of propolis, it enables to inhibit different cell lines and increase growth of normal cells. This indicates also that WEP contains the specific compounds with bioactivity against cell lines. Although propolis contain different number of compounds it is clear that WEP has enough biological compounds useful for the treatment of some diseases, medical and related applications.
蜂胶的水提取物（WEP）可以抑制不同细胞系的生长，如 McCoy、HeLa、SP2/0、HEp-2 和 BHK21，同时刺激正常细胞系如人淋巴细胞、大鼠肾、大鼠肝和大鼠脾的生长。在这些实验中，将 1 和 2 毫克的 WEP 添加到 1 毫升含有 5%FCS 的 RPMI 培养基中。通过台盼蓝染料排斥试验和 MTT 测定评估蜂胶处理和无蜂胶细胞的细胞计数和细胞活力。结果表明，对于 McCoy、HeLa、SP20、HEp-2 和 BHK21 细胞系，蜂胶的水提取物可以抑制细胞生长以及细胞大小的减小。相比之下，相同数量的 WEP 可以刺激正常细胞的生长，最高可达 60%，而细胞系的浓度相同。因此，我们的研究表明，尽管 WEP 仅由蜂胶的可溶性部分组成，但它能够抑制不同的细胞系并增加正常细胞的生长。这也表明 WEP 含有对细胞系具有生物活性的特定化合物。尽管蜂胶含有不同数量的化合物，但很明显 WEP 具有足够的用于治疗某些疾病、医学和相关应用的生物化合物。
