Frajese Giovanni Vanni, Benvenuto Monica, Mattera Rosanna, Giampaoli Saverio, Ambrosin Elena, Bernardini Roberta, Giganti Maria Gabriella, Albonici Loredana, Dus Ivan, Manzari Vittorio, Modesti Andrea, Mattei Maurizio, Bei Roberto
Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", 00135 Rome, Italy.
Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", 00133 Rome, Italy.
Evid Based Complement Alternat Med. 2018 Sep 26;2018:4753507. doi: 10.1155/2018/4753507. eCollection 2018.
Electrochemical reduced water (ERW) has been proposed to have beneficial effects on human health due to its rich content of H and the presence of platinum nanoparticles with antioxidant effects. Many studies have demonstrated that ERW scavenging properties are able to reduce the damage caused by oxidative stress in different experimental models. Although few studies have been reported, it has been demonstrated that ERW may display anticancer effects by induction of tumor cells apoptosis and reduction of both angiogenesis and inflammation. In this study, we show that ERW treatment of MCF-7, MDA-MB-453, and mouse (TUBO) breast cancer cells inhibited cell survival in a time-dependent fashion. ERW decreased ErbB2/ expression and impaired pERK1/ERK2 and AKT phosphorylation in breast cancer cells. In addition, ERW treatment induced apoptosis of breast cancer cell lines independently of the status of p53 and ER and PR receptors. Our results showed that ERW treatment of transgenic BALB-T mice delayed the development of mammary tumors compared to the control. In addition, ERW induced a significant prolongation of tumor-free survival and a reduction in tumor multiplicity. Overall, these results suggest a potential beneficial role of ERW in inhibiting cancer cells growth.
由于电化学还原水（ERW）富含氢且存在具有抗氧化作用的铂纳米颗粒，因此有人提出它对人体健康有益。许多研究表明，在不同的实验模型中，ERW的清除特性能够减少氧化应激造成的损害。尽管报道的研究较少，但已证明ERW可能通过诱导肿瘤细胞凋亡以及减少血管生成和炎症来发挥抗癌作用。在本研究中，我们发现用ERW处理MCF-7、MDA-MB-453和小鼠（TUBO）乳腺癌细胞会以时间依赖性方式抑制细胞存活。ERW降低了乳腺癌细胞中ErbB2的表达，并损害了pERK1/ERK2和AKT的磷酸化。此外，ERW处理可诱导乳腺癌细胞系凋亡，且与p53以及雌激素受体（ER）和孕激素受体（PR）的状态无关。我们的结果表明，与对照组相比，用ERW处理转基因BALB-T小鼠可延缓乳腺肿瘤的发展。此外，ERW可显著延长无瘤生存期并减少肿瘤的多发性。总体而言，这些结果表明ERW在抑制癌细胞生长方面具有潜在的有益作用。
