组织培养：未实现的潜力。
Tissue culture: the unrealized potential.
机构信息
A and G Pharmaceutical Corp., 27 Cedar St., Wenham, MA, 01984, USA,
出版信息
Cytotechnology. 2008 Jun;57(2):111-4. doi: 10.1007/s10616-007-9109-9. Epub 2007 Dec 11.
Lack of differentiated functions of the tissue of origin in tissue culture thought to be due to dedifferentiation was shown to be due to selective overgrowth of fibroblasts. Enrichment culture techniques, (alternate animal and culture passage), designed to give the functionally differentiated cells selective advantage over the fibroblasts resulted in a large number of functionally differentiated clonal strains. Thus the dogma of dedifferentiation was destroyed. It is proposed to substitute the dedifferentiation hypothesis with the hypothesis that cells in culture accurately represent cells in vivo without the complex in vivo environment. With the development of hormonally defined media, combined with functionally differentiated clonal cell lines, the potential of tissue culture studies is greatly augmented. Hormonal responses and dependencies can be discovered in culture and the discovery of dependencies of cancer cells has led to a new rationale for therapy.
在组织培养中，由于去分化，组织起源的功能分化缺失，这被认为是由于成纤维细胞的选择性过度生长。富集培养技术（交替使用动物和培养传代）旨在使功能分化细胞相对于成纤维细胞具有选择性优势，从而产生了大量具有功能分化的克隆株。因此，去分化的假说被推翻了。建议用细胞在培养中准确地代表体内细胞而不存在复杂的体内环境的假说来替代去分化假说。随着激素定义培养基的发展，结合功能分化的克隆细胞系，组织培养研究的潜力大大增加。可以在培养中发现激素反应和依赖性，并且对癌细胞依赖性的发现为治疗提供了新的依据。
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