Wiegmann Vincent, Martinez Cristina Bernal, Baganz Frank
The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Applikon-Biotechnology BV, Heertjeslaan 2, 2629 JG, Delft, The Netherlands.
Biotechnol Lett. 2018 Jul;40(7):1029-1036. doi: 10.1007/s10529-018-2556-x. Epub 2018 Apr 24.
Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes.
A correlation between evaporation and the concentration of Na was found (R = 0.95) when using the 24-well-based miniature bioreactor system (micro-Matrix) for a batch culture with GS-CHO. Based on these results, a method was developed to counteract evaporation with periodic water additions based on measurements of the Na concentration. Implementation of this method resulted in a reduction of the relative liquid loss after 15 days of a fed-batch cultivation from 36.7 ± 6.7% without volume corrections to 6.9 ± 6.5% with volume corrections.
A procedure was established to indirectly measure evaporation through a correlation with the level of Na ions in solution and deriving a simple formula to account for liquid losses.
建立一种间接测量基于微孔板的细胞培养系统中蒸发量的方法，并证明所提出的方法能够补偿补料分批培养过程中的液体损失。
当使用基于24孔的微型生物反应器系统（微基质）对GS-CHO进行分批培养时，发现蒸发与Na浓度之间存在相关性（R = 0.95）。基于这些结果，开发了一种基于Na浓度测量结果定期加水以抵消蒸发的方法。实施该方法后，补料分批培养15天后的相对液体损失从无体积校正时的36.7±6.7%降低至有体积校正时的6.9±6.5%。
建立了一种通过与溶液中Na离子水平的相关性间接测量蒸发量并推导一个简单公式来计算液体损失的程序。
