生成分泌抗花生过敏原 Ara h1 抗体的小鼠-人杂交瘤细胞
Generation of mouse-human hybridomas secreting antibodies against peanut allergen Ara h1.
机构信息
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan,
出版信息
Cytotechnology. 2004 Sep;46(1):19-23. doi: 10.1007/s10616-005-1578-0. Epub 2005 Jun 16.
Abstract
Two clones of mouse-human hybridomas, secreting human monoclonal antibodies to a peanut allergen Ara h1, were generated from human peripheral blood lymphocytes transformed with Epstein--Barr virus, followed by cell fusion with mouse myeloma cells. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed antibody-binding sequences of Ara h1 protein.
摘要
两个分泌针对花生过敏原 Ara h1 的人源单克隆抗体的鼠-人杂交瘤细胞系，是通过用 Epstein--Barr 病毒转化的人外周血淋巴细胞，然后与小鼠骨髓瘤细胞融合产生的。利用多针装置合成的重叠肽进行表位分析，揭示了 Ara h1 蛋白的抗体结合序列。
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Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2.花生过敏原 Ara h1 的表位分析与人类单克隆 IgM 抗体 92-2。
Cytotechnology. 2010 Aug;62(4):307-11. doi: 10.1007/s10616-009-9243-7. Epub 2009 Dec 19.
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Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells.用人类 B 淋巴细胞母细胞来源的寡克隆 IgM 抗体分析花生过敏原 Ara h1 的表位。
Cytotechnology. 2008 Jun;57(2):177-80. doi: 10.1007/s10616-008-9142-3. Epub 2008 Apr 11.
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