Centre de Formation aux Biotechnologies, 06402, Cannes, France.
Cytotechnology. 2001 Mar;35(2):93-100. doi: 10.1023/A:1017570815169.
The authors describe a novel spectrofluorometric technique based on double-labelled fluorescence imaging using immunoconjugates labelled with fluorochromes. Following isolation and characterization, cells are seeded on the surface of disks of the material(s) to be tested. After application of a primary antibody and an antibody bearing a fluorochrome, the signal emitted by the molecules in the extracellular matrix on the surface of the test disks is measured by spectrofluorimetry. Measurement is thus independent of the surface characteristics of the test material. Measured values are compared with pre-established standard curves. This technique facilitates determination of the characteristic molecules expressed by a given cell type,thus allowing accurate evaluation of the response of pertinent biological samples to implantable biomaterials.
作者描述了一种基于免疫缀合物标记荧光团的双标记荧光成像的新型荧光光谱技术。在分离和表征后，将细胞接种在待测试材料的圆盘表面上。应用一抗和带有荧光团的抗体后，通过荧光光谱法测量测试盘表面细胞外基质中分子发出的信号。因此，测量与测试材料的表面特性无关。测量值与预先建立的标准曲线进行比较。该技术有助于确定特定细胞类型表达的特征分子，从而可以准确评估相关生物样品对可植入生物材料的反应。
