Geraghty R J, Capes-Davis A, Davis J M, Downward J, Freshney R I, Knezevic I, Lovell-Badge R, Masters J R W, Meredith J, Stacey G N, Thraves P, Vias M
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK.
CellBank Australia, Children's Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia.
Br J Cancer. 2014 Sep 9;111(6):1021-46. doi: 10.1038/bjc.2014.166. Epub 2014 Aug 12.
Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.
细胞系的错误鉴定以及微生物污染，如支原体污染，再加上遗传和表型的不稳定性，都是持续影响细胞培养的问题。其中许多问题若有必要的前瞻性是可以避免的，编写本指南是为了向该领域的新手以及从事教学工作的其他人提供必要信息，以提高他们对这些问题的认识，并使他们能够有效应对。本指南涵盖了细胞系的建立、获取、鉴定、冷冻保存、实验室间转移、微生物污染、特性描述、不稳定性和错误鉴定等领域。还就从人和动物组织获取细胞系时遵守当前法律和道德要求、设备的选择和维护以及如何处理可能出现的问题提供了建议。
