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培养细胞中的交叉污染。

Cross-contamination of cells in culture.

作者信息

Nelson-Rees W A, Daniels D W, Flandermeyer R R

出版信息

Science. 1981 Apr 24;212(4493):446-52. doi: 10.1126/science.6451928.

DOI:10.1126/science.6451928
PMID:6451928
Abstract

Lists are presented of references to all known publications describing cell properties that serve to characterize (i) known strains of HeLa and purported human cell lines indicated as HeLa contaminants, (ii) strains of human cell lines contaminated with human but non-HeLa cells, and (iii) strains of cells contaminated by cells from one or more other species. Frequencies of cell cross-contaminations are cited and references are presented to relatively simple techniques that could serve to detect such contamination.

摘要

文中列出了所有已知出版物的参考文献,这些文献描述了用于表征以下细胞特性的内容:(i) 已知的海拉细胞系以及被指出作为海拉细胞污染物的所谓人类细胞系;(ii) 被人类但非海拉细胞污染的人类细胞系;(iii) 被来自一种或多种其他物种的细胞污染的细胞系。文中引用了细胞交叉污染的频率,并提供了可用于检测此类污染的相对简单技术的参考文献。

相似文献

1
Cross-contamination of cells in culture.培养细胞中的交叉污染。
Science. 1981 Apr 24;212(4493):446-52. doi: 10.1126/science.6451928.
2
HeLa cultures defined.已定义的海拉细胞培养物。
Science. 1976 Jan 9;191(4222):96-8. doi: 10.1126/science.1246601.
3
Enzyme polymorphisms as genetic signatures in human cell cultures.酶多态性作为人类细胞培养中的遗传特征。
Science. 1977 Mar 25;195(4284):1345-8. doi: 10.1126/science.841332.
4
Species identity of insect cell lines.昆虫细胞系的物种鉴定。
In Vitro. 1972 Mar-Apr;7(5):313-22. doi: 10.1007/BF02661721.
5
G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells.16种连续传代的人肿瘤细胞系的葡萄糖-6-磷酸脱氢酶(G-6-PD)和磷酸葡萄糖变位酶(PGM)表型。排除HeLa细胞交叉污染和污染的证据。
Hum Hered. 1971;21(3):238-41. doi: 10.1159/000152408.
6
[The problem of contamination of transplantable cell lines by HeLa cells].[可移植细胞系被海拉细胞污染的问题]
Vopr Virusol. 1977 Mar-Apr(2):246-51.
7
[Glucose-6-phosphate dehydrogenase isoenzyme in transplantable human cell cultures].[可移植人细胞培养物中的葡萄糖-6-磷酸脱氢酶同工酶]
Dokl Akad Nauk SSSR. 1974 Aug 2;217(5):1191-3.
8
[Isoenzyme characteristics of glucose-6-phosphage dehydrogenase and lactate dehydrogenase of continuous cells from the collection of the Sverdlovsk Research Institute of Virus Infections].[斯维尔德洛夫斯克病毒感染研究所馆藏连续传代细胞的葡萄糖-6-磷酸脱氢酶和乳酸脱氢酶同工酶特性]
Vopr Virusol. 1976 Sep-Oct(5):620-3.
9
Isozymic variants as genetic markers in somatic cell populations in vitro.同工酶变体作为体外体细胞群体中的遗传标记。
Natl Cancer Inst Monogr. 1968 Dec;29:9-13.
10
[Contamination of continuous cell lines].[连续细胞系的污染]
Vopr Virusol. 1980 Jul-Aug(4):432-9.

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