Abraham S K
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Mutat Res. 1991 Feb;262(2):109-14. doi: 10.1016/0165-7992(91)90115-k.
The mouse bone marrow micronucleus test was carried out to evaluate the possible inhibitory effects of 3 doses (125, 250 and 500 mg/kg) of standard instant coffee on the in vivo genotoxicity of 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BP), aflatoxin B1 (AFB1) and urethane (UR). Coffee was orally administered twice, 2 and 20 h before the carcinogens were injected intraperitoneally. From the results obtained, it was evident that the administration of 250 and 500 mg coffee/kg body weight could significantly inhibit the in vivo genotoxicity of these carcinogens. A linear dose response was observed for the inhibitory effect of coffee. Furthermore, inhibition of genotoxicity by coffee was observed in bone marrow cells which were sampled at 6-h intervals (48, 54, 60, 66 and 72 h) from the time of peak induction of micronuclei by DMBA.
进行小鼠骨髓微核试验，以评估3种剂量（125、250和500 mg/kg）的标准速溶咖啡对7,12-二甲基苯并[a]蒽（DMBA）、苯并[a]芘（BP）、黄曲霉毒素B1（AFB1）和氨基甲酸乙酯（UR）体内遗传毒性的可能抑制作用。咖啡在腹腔注射致癌物前2小时和20小时口服给药两次。从获得的结果来看，显然给予250和500 mg咖啡/千克体重可显著抑制这些致癌物的体内遗传毒性。观察到咖啡的抑制作用呈线性剂量反应。此外，在从DMBA诱导微核达到峰值时起每隔6小时（48、54、60、66和72小时）采集的骨髓细胞中观察到咖啡对遗传毒性的抑制作用。
