Moiteiro Cristina, Gaspar Helena, Rodrigues Ana I, Lopes João F, Carnide Valdemar
Instituto Nacional de Engenharia, Tecnologia e Inovação I.P., DTIQ, Lisboa, Portugal.
J Sep Sci. 2008 Dec;31(21):3683-7. doi: 10.1002/jssc.200800383.
A HPLC method was developed for the simultaneous identification of Reseda luteola L. (weld) flavonoids and quantification of the main compounds responsible for the yellow color. This method was applied to a large number of wild Portuguese weld to evaluate its potential application as dyestuff for textile factories, as a substitute for the synthetic dyes currently used. Portuguese weld dyestuff content ranged between 1.04 and 5.87%, corresponding to a wide variation of the flavonoids amount (1.39-9.04%). Luteolin 4'-O-glucoside was found for the first time in R. luteola, but kaempferol, isorhamnetin, and their glycosides were not detected in the Portuguese specimens.
开发了一种高效液相色谱法，用于同时鉴定淡黄木犀草（焊接用）黄酮类化合物，并对造成黄色的主要化合物进行定量分析。该方法应用于大量葡萄牙野生淡黄木犀草，以评估其作为纺织厂染料的潜在应用，作为目前使用的合成染料的替代品。葡萄牙淡黄木犀草染料含量在1.04%至5.87%之间，这对应着黄酮类化合物含量的广泛变化（1.39 - 9.04%）。首次在淡黄木犀草中发现了木犀草素4'-O-葡萄糖苷，但在葡萄牙样本中未检测到山奈酚、异鼠李素及其糖苷。
