Hamaguchi Yasuhito, Fujimoto Manabu, Matsushita Takashi, Hasegawa Minoru, Takehara Kazuhiko, Sato Shinichi
Department of Dermatology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
J Rheumatol. 2008 Dec;35(12):2363-71. doi: 10.3899/jrheum.080340. Epub 2008 Nov 1.
To examine serum concentrations of insulin-like growth factor (IGF-1) and IGF binding protein (IGFBP-3), a major carrier protein for IGF-1, in patients with systemic sclerosis (SSc); and to relate the results to clinical features in SSc.
Serum IGF-1 and IGFBP-3 levels in 92 Japanese patients with SSc were measured by ELISA. Expression of IGF-1 and IGFBP-3 messenger RNA (mRNA) in the skin was quantified by real-time reverse transcription-polymerase chain reaction.
Serum IGF-1 and IGFBP-3 levels were significantly elevated in patients with SSc compared with patients with systemic lupus erythematosus or healthy controls. IGF-1 levels were higher in patients with diffuse cutaneous SSc (dcSSc) than in patients with limited cutaneous SSc (lcSSc). Patients with increased IGF-1 levels had more severe skin involvement and pulmonary fibrosis. IGF-1 mRNA was upregulated in the affected skin of patients with SSc. There were no significant differences in serum IGFBP-3 levels between dcSSc and lcSSc. IGFBP-3 levels were not associated with skin thickness and pulmonary fibrosis. Patients with increased IGF-1 or IGFBP-3 had lower frequency of telangiectasia than patients with normal levels.
These results suggest that both IGF-1 and IGFBP-3 are involved in the development of SSc. The role of IGF-1 appears to be different from that of IGFBP-3.
检测系统性硬化症（SSc）患者血清中胰岛素样生长因子（IGF-1）及其主要载体蛋白胰岛素样生长因子结合蛋白（IGFBP-3）的浓度，并将结果与SSc的临床特征相关联。
采用酶联免疫吸附测定法（ELISA）检测92例日本SSc患者血清中的IGF-1和IGFBP-3水平。通过实时逆转录-聚合酶链反应对皮肤中IGF-1和IGFBP-3信使核糖核酸（mRNA）的表达进行定量分析。
与系统性红斑狼疮患者或健康对照相比，SSc患者血清中的IGF-1和IGFBP-3水平显著升高。弥漫性皮肤型SSc（dcSSc）患者的IGF-1水平高于局限性皮肤型SSc（lcSSc）患者。IGF-1水平升高的患者皮肤受累和肺纤维化更为严重。SSc患者受累皮肤中IGF-1 mRNA上调。dcSSc和lcSSc患者血清IGFBP-3水平无显著差异。IGFBP-3水平与皮肤厚度和肺纤维化无关。IGF-1或IGFBP-3升高的患者毛细血管扩张的发生率低于水平正常的患者。
这些结果表明IGF-1和IGFBP-3均参与了SSc的发病过程。IGF-1的作用似乎与IGFBP-3不同。
