Rozkiewicz Dorota I, Kraan Yvonne, Werten Marc W T, de Wolf Frits A, Subramaniam Vinod, Ravoo Bart Jan, Reinhoudt David N
Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Chemistry. 2006 Aug 16;12(24):6290-7. doi: 10.1002/chem.200501554.
We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive "islands", the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.
我们描述了一种通过微接触印刷将亲细胞蛋白共价固定的直接方法，该方法可用于在底物上对细胞进行图案化处理。利用亚胺化学方法，将亲细胞蛋白以微图案形式印刷在反应性自组装单分子层上。通过氨基端基单分子层与对苯二甲醛之间的反应，在玻璃或金表面获得醛基端基单分子层。醛基单分子层用作底物，通过使用氧化聚二甲基硅氧烷（PDMS）印章对生物工程化的类胶原蛋白进行直接微接触印刷。将蛋白质固定到粘性“岛”中后，其余区域用氨基聚乙二醇封闭，该物质形成一层抗细胞粘附的层。将人恶性癌细胞（HeLa）接种并培养在图案化的底物上。发现这些细胞选择性地粘附并铺展在蛋白质岛上，而避开聚乙二醇（PEG）区域。这些发现说明了微接触印刷作为一种在表面定位蛋白质的方法的重要性，并展示了可控表面化学在指导细胞粘附方面的应用范围。
