Gu Ying, Deng Zhiping, Paredez Alexander R, DeBolt Seth, Wang Zhi-Yong, Somerville Chris
Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA 94305, USA.
Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):18064-9. doi: 10.1073/pnas.0808652105. Epub 2008 Nov 11.
Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.
新翻译的微管蛋白分子会与新生链结合伴侣发生一系列复杂的相互作用，这些伴侣包括预折叠蛋白（PFD）和含伴侣蛋白的TCP-1（CCT）。通过筛选对oryzalin超敏的突变体，我们鉴定出了拟南芥中几个在PFD亚基上有损伤的突变体。pfd6-1突变体表现出一系列微管缺陷，包括对oryzalin超敏、细胞分裂缺陷、皮层阵列组织缺陷以及微管动态性缺陷。与表型分析一致，蛋白质组学分析表明pfd6-1中几种微管蛋白异构体减少。这些结果支持了微管功能严重依赖于微管蛋白绝对量的观点。
