Patel Pinal C, Giljohann David A, Seferos Dwight S, Mirkin Chad A
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113, USA.
Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17222-6. doi: 10.1073/pnas.0801609105.
We have designed a heterofunctionalized nanoparticle conjugate consisting of a 13-nm gold nanoparticle (Au NP) containing both antisense oligonucleotides and synthetic peptides. The synthesis of this conjugate is accomplished by mixing thiolated oligonucleotides and cysteine-terminated peptides with gold nanoparticles in the presence of salt, which screens interactions between biomolecules, yielding a densely functionalized nanomaterial. By controlling the stoichiometry of the components in solution, we can control the surface loading of each biomolecule. The conjugates are prepared easily and show perinuclear localization and an enhanced gene regulation activity when tested in a cellular model. This heterofunctionalized structure represents a new strategy for preparing nanomaterials with potential therapeutic applications.
我们设计了一种异功能化纳米颗粒缀合物，它由一个13纳米的金纳米颗粒（Au NP）组成，该金纳米颗粒同时含有反义寡核苷酸和合成肽。这种缀合物的合成是通过在盐存在的情况下，将硫醇化寡核苷酸和半胱氨酸末端的肽与金纳米颗粒混合来完成的，盐可以屏蔽生物分子之间的相互作用，从而产生一种功能密集化的纳米材料。通过控制溶液中各组分的化学计量比，我们可以控制每种生物分子的表面负载量。这些缀合物易于制备，并且在细胞模型中测试时显示出核周定位和增强的基因调控活性。这种异功能化结构代表了一种制备具有潜在治疗应用的纳米材料的新策略。
