Luo Haitao, Jiang Bing-Hua, King Sarah M, Chen Yi Charlie
Natural Science Division, Alderson-Broaddus College, Philippi, WV 26416, USA.
Nutr Cancer. 2008;60(6):800-9. doi: 10.1080/01635580802100851.
Dietary flavonoids have been shown to be protective against various types of cancers. Here we studied the effects of 12 different flavonoids and other substances on cell proliferation and VEGF expression in human ovarian cancer cells, OVCAR-3. Cell growth was determined to pinpoint the best time for drug treatment. By LDH assay, no cytotoxicity was observed for OVCAR-3 cells with all 12 chemicals except mevinolin. Six flavonoids, including apigenin, taxifolin, luteolin, quercetin, genistein, and kaempferol, were shown to inhibit the ovarian cancer cell growth in a dose-dependent manner. From both RT-qPCR and ELISA results, all flavonoids have shown varied degrees of inhibition in VEGF expression. Taxifolin and naringin showed the least inhibition effect. They both lack a double bond in the second ring structure that may be important in inhibiting VEGF expression. The rank order of VEGF protein secretion inhibitory potency was genistein > kaempferol > apigenin > quercetin > tocopherol > luteolin > cisplatin > rutin > naringin > taxifolin. Genistein, quercetin, and luteolin have shown strong inhibition to cell proliferation and VEGF expression of human ovarian cancer cells, and they show promising in the prevention of ovarian cancers.
膳食类黄酮已被证明对多种类型的癌症具有保护作用。在此，我们研究了12种不同的类黄酮及其他物质对人卵巢癌细胞OVCAR-3细胞增殖和VEGF表达的影响。通过测定细胞生长来确定最佳药物治疗时间。通过乳酸脱氢酶（LDH）测定法，发现除美伐他汀外，其余12种化学物质对OVCAR-3细胞均未观察到细胞毒性。包括芹菜素、紫杉叶素、木犀草素、槲皮素、染料木黄酮和山奈酚在内的6种类黄酮均呈剂量依赖性地抑制卵巢癌细胞生长。从逆转录定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）结果来看，所有类黄酮均对VEGF表达表现出不同程度的抑制作用。紫杉叶素和柚皮苷的抑制作用最小。它们在第二个环结构中都缺少一个双键，而这个双键可能对抑制VEGF表达很重要。VEGF蛋白分泌抑制效力的排序为：染料木黄酮＞山奈酚＞芹菜素＞槲皮素＞生育酚＞木犀草素＞顺铂＞芦丁＞柚皮苷＞紫杉叶素。染料木黄酮、槲皮素和木犀草素对人卵巢癌细胞的细胞增殖和VEGF表达均表现出强烈抑制作用，在预防卵巢癌方面具有良好前景。
