Spasojević V
Dev Biol Stand. 1977;34:197-205.
The toxicity of 201 Bordetella pertussis strains isolated in Belgrade has been studied. The strains were grown on a modification of Cohen and Wheeler's medium. LD50 of examined strains ranging between 1.06 and 1.95 billion bacteria in 35 (17.41%), between 2.04 and 4.83 billion bacteria in 92 (45.77%) and between 5.31 and 7.79 billion bacteria in 46 (22.88%) were found. Low toxic cultures of B. pertussis strains with LD50 ranging between 9.0 and 14.1 billion bacteria in 28 (13.43%) were obtained. The culture of the strains with low toxicity was 7 to 14 times more toxic than those prepared from the strains with low toxicity. In the cultures of 51 B. pertussis strains, toxicity for mice was not correlated with the number of viable cells. The toxic substances which influenced the loss of mouse weight were found in the culture filtrate of B. pertussis. A considerable detoxifying effect of heating at 56 degrees C for 30 min on liquid cultures prepared from highly toxic strains of B. pertussis was observed. The susceptibility of two different strains of mice for pertussis vaccine has been examined. The Albany strain of mice was less susceptible to toxicity control of the vaccine by mouse-weight-gain test than the Torlak strain.
对在贝尔格莱德分离出的201株百日咳博德特氏菌菌株的毒性进行了研究。这些菌株在改良的科恩和惠勒培养基上生长。在所检测的菌株中，半数致死剂量（LD50）在10.6亿至19.5亿个细菌之间的有35株（17.41%），在20.4亿至48.3亿个细菌之间的有92株（45.77%），在53.1亿至77.9亿个细菌之间的有46株（22.88%）。还获得了28株（13.43%）LD50在90亿至141亿个细菌之间的低毒百日咳博德特氏菌菌株培养物。低毒菌株的培养物毒性比由低毒菌株制备的培养物高7至14倍。在51株百日咳博德特氏菌菌株的培养物中，对小鼠的毒性与活细胞数量无关。在百日咳博德特氏菌的培养滤液中发现了影响小鼠体重减轻的有毒物质。观察到对由高毒百日咳博德特氏菌菌株制备的液体培养物在56℃加热30分钟有相当大的解毒作用。检测了两种不同品系小鼠对百日咳疫苗的易感性。与托尔拉克品系小鼠相比，奥尔巴尼品系小鼠通过小鼠体重增加试验对疫苗毒性控制的易感性较低。
