Wu Wei, Zhang Qing, Zhu Yanming, Lam Hon-Ming, Cai Zongwei, Guo Dianjing
Department of Biology, The Chinese University of Hong Kong, Shatin, N. T., SAR, China.
J Agric Food Chem. 2008 Dec 10;56(23):11132-8. doi: 10.1021/jf8024024.
High-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) and HPLC-ESI-MS(n) analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds. Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties. Principle component analysis clearly demonstrated that it is possible to use secondary metabolites, for example, isoflavones and saponins, to discriminate between closely related soybean genotypes. Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance. These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.
采用高效液相色谱 - 紫外 - 电喷雾电离质谱（HPLC - UV - ESI - MS）和HPLC - ESI - MS(n)分析方法对大豆种子中的异黄酮和皂苷进行代谢谱分析及同时鉴定。比较靶向代谢谱分析揭示了盐敏感型和耐盐型大豆品种之间代谢物组成的显著差异。主成分分析清楚地表明，利用次生代谢物，例如异黄酮和皂苷，能够区分亲缘关系相近的大豆基因型。染料木苷和B组皂苷被鉴定为与耐盐性相关的关键次生代谢物。这些单个代谢物可能为植物的耐盐性和适应性提供更多见解。
