Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
Sci Rep. 2017 Feb 13;7:42478. doi: 10.1038/srep42478.
The isoflavone profiles of seeds of various soybean genotypes with different levels of shade tolerance at the seedling stage were investigated. High-performance liquid chromatography (HPLC) was used to quantify 12 isoflavones, and the data were analyzed using a multivariate statistical analysis. Combined with field experimental data and an orthogonal partial least-squares discriminant analysis (OPLS-DA), several aglycones (genistein (GE), daidzein (DE), and glycitein (GLE)) were selected and identified as key compounds involved in the shade tolerance of soybean seedlings. Additional correlation analysis and laboratory shading stress experiments with soybean seedlings also confirmed the function of these selected isoflavones, especially GE, in the discrimination of soybean seedlings with different levels of shade tolerance. Furthermore, the structure-antioxidant activity relationships between a range of isoflavones and the plant shade-tolerance mechanism are discussed. Targeted metabolomic analyses of isoflavones could reveal the diversity of shade tolerance in soybean seedlings, thus contributing to the breeding of excellent soybean varieties.
本研究调查了不同耐荫性水平的大豆品种幼苗期种子的异黄酮谱。采用高效液相色谱法（HPLC）定量分析了 12 种异黄酮，并用多元统计分析对数据进行了分析。结合田间实验数据和正交偏最小二乘判别分析（OPLS-DA），选择了几种苷元（染料木黄酮（GE）、大豆苷元（DE）和黄豆苷元（GLE）），并将其鉴定为参与大豆幼苗耐荫性的关键化合物。此外，对大豆幼苗的相关性分析和实验室遮荫胁迫实验也证实了这些选定的异黄酮，尤其是 GE，在区分不同耐荫性水平的大豆幼苗中的作用。进一步讨论了一系列异黄酮的结构-抗氧化活性关系与植物耐荫性机制之间的关系。异黄酮的靶向代谢组学分析可以揭示大豆幼苗耐荫性的多样性，从而有助于培育优良的大豆品种。
