State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi University of Chinese Medicine, Xi'an, 712046, People's Republic of China.
The Key Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xi'an, 712046, People's Republic of China.
Drug Des Devel Ther. 2021 Sep 23;15:4025-4042. doi: 10.2147/DDDT.S330222. eCollection 2021.
is a natural medicinal and food plant that is rich in triterpenoid saponins with hypoglycaemic, antioxidant, hepatoprotective, anti-gastric ulcer and anti-inflammatory effects. This study has significance in terms of the antioxidant, anti-aging and organ protective effects of total saponins (TSAT) in D-galactose-induced aging rats.
The saponin composition of TSAT was determined and quantified by high performance liquid chromatography (HPLC). We consolidated the antioxidant and enzyme inhibitory activities of TSAT in vitro and assessed the effects of TSAT on daily mobility, body weight, behaviour, organ indices, oxidation-related indices and pathological changes in aging rats.
In vitro experiments showed that TSAT had a scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), tyrosinase, hydroxyl radicals (HO•) and superoxide radicals (•O) and was closely related to the dose of TSAT. In vivo experiments showed that after 8 weeks of continuous gavage administration, the rats gradually recovered their body weight, daily activity ability, learning and memory ability and organ index and effectively improved D-gal-induced organ injury. Specifically, TSAT significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) and significantly decreased malondialdehyde (MDA) levels in the serum, brain, heart, lung, spleen and kidney of aging rats compared to the model group. In addition, TSAT significantly inhibited the D-gal-induced upregulation of hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. The histopathological results showed that TSAT reversed D-gal-induced damage to the brain, heart, lung, kidney, liver and spleen to varying degrees.
TSAT is a high-quality natural product with antioxidant and anti-aging properties that can alleviate D-gal-induced aging damage in rats.
是一种天然的药用和食用植物，富含具有降血糖、抗氧化、保肝、抗胃溃疡和抗炎作用的三萜皂苷。本研究对于 D-半乳糖诱导衰老大鼠中天麻总皂苷（TSAT）的抗氧化、抗衰老和器官保护作用具有重要意义。
采用高效液相色谱法（HPLC）测定和定量分析 TSAT 的皂苷组成。我们综合评价了 TSAT 的体外抗氧化和酶抑制活性，并评估了 TSAT 对 D-半乳糖诱导衰老大鼠日常活动能力、体重、行为、器官指数、氧化相关指标和病理变化的影响。
体外实验表明，TSAT 对 1,1-二苯基-2-苦基肼基（DPPH）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、酪氨酸酶、羟基自由基（HO•）和超氧自由基（•O）具有清除作用，且与 TSAT 的剂量密切相关。体内实验表明，连续灌胃 8 周后，大鼠逐渐恢复体重、日常活动能力、学习记忆能力和器官指数，有效改善了 D-半乳糖诱导的器官损伤。具体来说，TSAT 显著提高了衰老大鼠血清、脑、心、肺、脾和肾中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（T-AOC）的水平，显著降低了丙二醛（MDA）水平。此外，TSAT 显著抑制了 D-半乳糖诱导的肝丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平的上调。组织病理学结果表明，TSAT 不同程度地逆转了 D-半乳糖诱导的脑、心、肺、肾、肝和脾损伤。
TSAT 是一种具有抗氧化和抗衰老特性的优质天然产物，可减轻 D-半乳糖诱导的大鼠衰老损伤。
