Morimoto Yuya, Tan Wei-Heong, Takeuchi Shoji
Center for International Research on MicroMechatronics (CIRMM), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Biomed Microdevices. 2009 Apr;11(2):369-77. doi: 10.1007/s10544-008-9243-y.
This paper describes a three-dimensional microfluidic axisymmetric flow-focusing device (AFFD) fabricated using stereolithography. Using this method, we can fabricate AFFDs rapidly and automatically without cumbersome alignment needed in conventional methods. The AFFDs are able to be fabricated reproducibly with a micro-sized orifice of diameter around 250 mum. Using this device, we are able to produce monodisperse water-in-oil (W/O) droplets with a coefficient of variation (CV) of less than 4.5%, W/O droplets with encapsulated microbes (CV < 4.9%) and oil-in-water (O/W) droplets (CV < 3.2%) without any surface modifications. The diameter of these droplets range from 54 to 244 mum with respect to the flow rate ratio of the fluids used; these results are in good agreement with theoretical behavior. For applications of the AFFD, we demonstrate that these devices can be used to produce double emulsions and monodisperse hydrogel beads.
本文描述了一种使用立体光刻技术制造的三维微流控轴对称流动聚焦装置（AFFD）。使用这种方法，我们可以快速、自动地制造AFFD，而无需传统方法中繁琐的对准操作。AFFD能够以直径约250μm的微尺寸孔口可重复制造。使用该装置，我们能够在不进行任何表面改性的情况下，生产变异系数（CV）小于4.5%的单分散油包水（W/O）液滴、包封微生物的W/O液滴（CV<4.9%）和水包油（O/W）液滴（CV<3.2%）。这些液滴的直径根据所用流体的流速比在54至244μm范围内；这些结果与理论行为高度吻合。对于AFFD的应用，我们证明这些装置可用于生产双重乳液和单分散水凝胶珠。
