Lazzeri Paul A, Jones Huw D
Agrasys S.L., Barcelona, Spain.
Methods Mol Biol. 2009;478:3-20. doi: 10.1007/978-1-59745-379-0_1.
Ever since the first developments in plant transformation technology using model plant species in the early 1980s, there has been a body of plant science research devoted to adapting these techniques to the transformation of crop plants. For some crop species progress was relatively rapid, but in other crop groups such as the small grain cereals, which were not readily amenable to culture in vitro and were not natural hosts to Agrobacterium, it has taken nearly two decades to develop reliable and robust transformation methods.In the following chapters of this book, transformation procedures for small grain cereals are presented, together with methods for gene and protein expression and the characterization of transgenic plants. In this introductory chapter we try to put these later chapters into context, giving an overview of the development of transformation technology for small grain cereals, discussing some of the pros and cons of the techniques and what limitations still exist.
自20世纪80年代初利用模式植物物种开展植物转化技术的首次研究以来，一直有一批植物科学研究致力于使这些技术适用于作物的转化。对于一些作物品种，进展相对较快，但在其他作物类别中，如不易进行离体培养且不是农杆菌天然宿主的小粒谷物，开发可靠且强大的转化方法花费了近二十年时间。在本书接下来的章节中，将介绍小粒谷物的转化程序，以及基因和蛋白质表达方法以及转基因植物的表征。在这一引言章节中，我们试图将后面的章节置于背景之中，概述小粒谷物转化技术的发展，讨论这些技术的一些优缺点以及仍然存在哪些局限性。
