Toyosawa S, Ogawa Y, Chang C K, Hong S S, Yagi T, Kuwahara H, Wakasa K, Sakurai M
Department of Oral Pathology, Osaka University Faculty of Dentistry, Japan.
Virchows Arch A Pathol Anat Histopathol. 1991;418(3):255-61. doi: 10.1007/BF01606064.
Using routinely processed, paraffin-embedded tissue specimens, osteoclast-like giant cells in giant cell tumour of bone (GCT), chondroblastoma, osteoblastoma and osteoblastic osteosarcoma were examined histochemically for osteoclast-specific enzymes tartrate-resistant acid phosphatase (TRAP) and carbonic anhydrase isoenzyme II (CA-II). Osteoclast-like giant cells and some mononuclear cells possessed TRAP activity. These were further classified with respect to CA-II immunoreactivity, i.e. cells with CA-II were seen in GCT and chondroblastoma, while those in osteoblastoma and osteoblastic osteosarcoma were negative for CA-II. All the cellular components in malignant fibrous histiocytoma and various extraosseous inflammatory lesions including malignant giant cells and macrophage polykaryons were negative for both TRAP and CA-II. These results indicate that osteoclast-like giant cells in GCT, chondroblastoma, osteoblastoma and osteoblastic osteosarcoma are all osteoclasts and generated by fusion of mononuclear cells with the same histochemical characteristics as osteoclast-like giant cells. The difference in CA-II immunoreactivity suggests the functional or maturational difference between osteoclast-like giant cells in GCT and chondroblastoma and those in osteoblastoma and osteosarcoma.
利用常规处理的石蜡包埋组织标本，对骨巨细胞瘤（GCT）、软骨母细胞瘤、成骨细胞瘤和骨母细胞性骨肉瘤中的破骨细胞样巨细胞进行组织化学检查，以检测破骨细胞特异性酶——抗酒石酸酸性磷酸酶（TRAP）和碳酸酐酶同工酶II（CA-II）。破骨细胞样巨细胞和一些单核细胞具有TRAP活性。根据CA-II免疫反应性对这些细胞进一步分类，即在GCT和软骨母细胞瘤中可见具有CA-II的细胞，而成骨细胞瘤和骨母细胞性骨肉瘤中的细胞CA-II呈阴性。恶性纤维组织细胞瘤以及包括恶性巨细胞和巨噬细胞多核体在内的各种骨外炎症性病变中的所有细胞成分，TRAP和CA-II均为阴性。这些结果表明，GCT、软骨母细胞瘤、成骨细胞瘤和骨母细胞性骨肉瘤中的破骨细胞样巨细胞均为破骨细胞，由与破骨细胞样巨细胞具有相同组织化学特征的单核细胞融合产生。CA-II免疫反应性的差异表明GCT和软骨母细胞瘤中的破骨细胞样巨细胞与成骨细胞瘤和骨肉瘤中的破骨细胞样巨细胞在功能或成熟度上存在差异。
