Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Biosens Bioelectron. 2009 Dec 15;25(4):889-95. doi: 10.1016/j.bios.2009.09.002. Epub 2009 Sep 6.
A practical glucose biosensor was developed by combining the intrinsic peroxidase-like activity of Fe(3)O(4) nanoparticles (Fe(3)O(4) NPs) and the anti-interference ability of the nafion film. Glucose oxidase (GOD) was simply mixed with Fe(3)O(4) NPs and cross-linked on the Pt electrode with chitosan (Cs) medium by glutaraldehyde, and then covered with a thin nafion film. The biosensor showed high sensitivity (11.54 microAcm(-2)mM(-1)), low detection limit (6 x10(-6)M), and good storage stability. A linear calibration plot was obtained in the wide concentration range from 6 x10(-6) to 2.2 x10(-3)M. The modified electrode could virtually eliminate the interference during the detection of glucose. Furthermore, the biosensor was successfully applied to detect glucose in human serum sample. This fabrication of glucose biosensor was of considerable interest due to its promise for simple procedure and optimizing conditions in practical application.
一种实用的葡萄糖生物传感器是通过结合 Fe(3)O(4) 纳米粒子（Fe(3)O(4) NPs）的固有过氧化物酶样活性和纳滤膜的抗干扰能力来开发的。葡萄糖氧化酶（GOD）与 Fe(3)O(4) NPs 简单混合，并通过戊二醛交联在壳聚糖（Cs）介质中的 Pt 电极上，然后用一层薄薄的纳滤膜覆盖。该生物传感器具有高灵敏度（11.54 μAcm(-2)mM(-1))、低检测限（6 x10(-6)M）和良好的储存稳定性。在从 6 x10(-6) 到 2.2 x10(-3)M 的宽浓度范围内获得了线性校准曲线。修饰电极可以有效地消除检测葡萄糖过程中的干扰。此外，该生物传感器已成功应用于检测人血清样品中的葡萄糖。由于其在实际应用中具有简单的程序和优化条件的潜力，因此这种葡萄糖生物传感器的制备具有很大的意义。
