Villeneuve L, Alberti L, Steghens J-P, Lancelin J-M, Mestas J-L
Hospices Civils de Lyon, Pôle d'Information Médicale, Evaluation et Recherche, Lyon F-69003, France.
Ultrason Sonochem. 2009 Mar;16(3):339-44. doi: 10.1016/j.ultsonch.2008.09.007. Epub 2008 Oct 10.
Water sonolysis leads to the formation of hydroxyl radicals (OH*). Various techniques are used to detect the OH* production and thus to assess the level of ultrasound-mediated cavitation generated in vitro. In this study, we used terephthalic acid (TA) as an OH* trap. This method is based on the fluorescent properties of hydroxyterephthalic acid (HTA) formed by the reaction of TA with OH* and used as an indicator of the degree of inertial cavitation caused. The experimental system is comprised mainly of a focused piezoelectric ultrasound transmitter and a measurement cell containing 1X PBS/TA diluted solution. In the first part, we aimed to characterize the most appropriate experimental conditions (TA dosimeter solution, irradiation time) in order to optimize the resulting HTA fluorescence values. Then, we could determine that the HTA production increased with the level of the cavitation phenomenon caused by the acoustic power from which OH* production may be estimated.
水超声分解会导致羟基自由基（OH*）的形成。人们使用各种技术来检测OH的产生，从而评估体外产生的超声介导空化的水平。在本研究中，我们使用对苯二甲酸（TA）作为OH捕获剂。该方法基于TA与OH反应形成的羟基对苯二甲酸（HTA）的荧光特性，并用作所引起的惯性空化程度的指标。实验系统主要由一个聚焦压电超声发射器和一个含有1X PBS/TA稀释溶液的测量池组成。在第一部分中，我们旨在确定最合适的实验条件（TA剂量计溶液、照射时间），以优化所得的HTA荧光值。然后，我们可以确定HTA的产生随着由声功率引起的空化现象水平的增加而增加，由此可以估计OH的产生。
