使用酶免疫测定法检测鼻咽抽吸物中的人偏肺病毒抗原。
Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay.
作者信息
Kukavica-Ibrulj Irena, Boivin Guy
机构信息
Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada.
出版信息
J Clin Virol. 2009 Jan;44(1):88-90. doi: 10.1016/j.jcv.2008.09.011. Epub 2008 Nov 17.
Abstract
BACKGROUND
Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups.
OBJECTIVE
To evaluate the performance of a rapid antigenic test for all hMPV genotypes.
STUDY DESIGN
Frozen nasopharyngeal aspirates from 93 individuals with ARTI were analyzed for the presence of hMPV antigens using an enzyme immunoassay (EIA, Biotrin Ltd.).
RESULTS
The hMPV EIA showed a sensitivity of 81%, a specificity of 100%, a positive predictive value of 100% and a negative predictive value of 77% compared to viral culture and RT-PCR.
CONCLUSION
The Biotrin hMPV EIA is a convenient alternative to cell culture for detection of hMPV with an excellent specificity.
摘要
背景
人偏肺病毒（hMPV）与各年龄组患者的急性呼吸道感染（ARTI）相关。
目的
评估针对所有hMPV基因型的快速抗原检测的性能。
研究设计
使用酶免疫测定法（EIA，Biotrin有限公司）分析来自93例急性呼吸道感染患者的冷冻鼻咽抽吸物中hMPV抗原的存在情况。
结果
与病毒培养和逆转录-聚合酶链反应相比，hMPV酶免疫测定法的灵敏度为81%，特异性为100%，阳性预测值为100%，阴性预测值为77%。
结论
Biotrin hMPV酶免疫测定法是一种用于检测hMPV的便捷替代细胞培养的方法，具有出色的特异性。
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