Rombaldi Fernanda, Cassini Carina, Salvador Mirian, Saffi Jenifer, Erdtmann Bernardo
Instituto de Biotecnologia, Universidade de Caxias do Sul, Rio Grande do Sul, Brasil.
Mutagenesis. 2009 Mar;24(2):143-8. doi: 10.1093/mutage/gen060. Epub 2008 Nov 14.
Twenty pharmacists and nurses handling anti-neoplastic drugs in a hospital were monitored during a working week, from Monday to Friday, in the morning (only on Monday) and afternoon (all days). Genotoxicity was analysed by the comet assay and the micronucleus (MN) test, while oxidative stress was analysed in serum by thiobarbituric acid reactive substances (TBARS) and by measurements of the antioxidant enzymes superoxide dismutase (Sod) and catalase (Cat). The exposed workers presented increased DNA damage levels by the comet assay as compared to the controls. The comet assay results have also shown significant positive correlation with the day of the week and with alcohol consumption. MN frequency was significantly higher in the exposed workers and presented noteworthy correlation with age and working time. In the oxidative stress parameters, only Cat presented a significant increase in the exposed group, considering all the samplings. However, TBARS data showed interesting results, considering the different sampling times; the exposed group presented a significant correlation with the working days and significantly higher results on Friday as compared to the controls and Monday morning. Monitoring occupational risk during a longer time, e.g. during a working week as done in this study, introduces additional aspects of risk behaviour, which can improve risk management. This study demonstrates the usefulness of evaluating oxidative stress also in genotoxic risk assessment since both events often result from the same factors.
在一周的工作日期间（周一至周五），对一家医院中20名处理抗肿瘤药物的药剂师和护士进行了监测，监测时间为上午（仅周一）和下午（所有日子）。通过彗星试验和微核（MN）试验分析遗传毒性，同时通过硫代巴比妥酸反应性物质（TBARS）以及对抗氧化酶超氧化物歧化酶（Sod）和过氧化氢酶（Cat）的测量来分析血清中的氧化应激。与对照组相比，通过彗星试验发现暴露组的DNA损伤水平有所增加。彗星试验结果还显示与星期几以及饮酒量呈显著正相关。暴露组的MN频率显著更高，并且与年龄和工作时间呈现出值得注意的相关性。在氧化应激参数方面，考虑到所有采样情况，仅Cat在暴露组中呈现出显著增加。然而，考虑到不同的采样时间，TBARS数据显示出有趣的结果；暴露组与工作日呈现出显著相关性，并且与对照组和周一上午相比，周五的结果显著更高。在更长的时间段内监测职业风险，例如像本研究中在一周的工作日期间进行监测，会引入风险行为的其他方面，这可以改善风险管理。这项研究证明了在遗传毒性风险评估中评估氧化应激的有用性，因为这两种情况通常由相同的因素导致。
