Lu H D, Chen G, Ts'o D Y, Roe A W
Dept of Psychology, 301 Wilson Hall, Vanderbilt University, Nashville, TN 37212, USA.
Neuroimage. 2009 Feb 1;44(3):636-46. doi: 10.1016/j.neuroimage.2008.10.007. Epub 2008 Oct 25.
In optical imaging experiments, it is often advantageous to map the field of view and to converge the eyes without electrophysiological recording. This occurs when limited space precludes placement of an electrode or in chronic optical chambers in which one may not want to introduce an electrode each session or for determining eye position in studies of ocular disparity response in visual cortex of anesthetized animals. For these purposes, we have developed a spot imaging method that can be conducted rapidly and repeatedly throughout an experiment. Using small 0.2 degrees -0.5 degrees spots, the extent of the imaged field of view is mapped by imaging cortical response to single spots, placed at different positions (0.2 degrees steps) in either the horizontal or vertical axes. By shifting the relative positions of two spots, one presented to each eye, eye convergence can be assessed to within 0.1 degrees resolution. Once appropriate eye alignment is determined, stimuli for further optical imaging procedures (e.g. imaging random dot stimuli for study of disparity responses) can then be confidently placed. This procedure can be quickly repeated throughout the experiment to ensure maintained eye alignment.
在光学成像实验中，在不进行电生理记录的情况下绘制视野并使眼睛会聚通常是有利的。当空间有限而无法放置电极时，或者在慢性光学室中，可能不想在每次实验时都插入电极，或者在研究麻醉动物视觉皮层的眼视差反应时确定眼睛位置时，就会出现这种情况。出于这些目的，我们开发了一种光斑成像方法，该方法可以在整个实验过程中快速且重复地进行。使用小至0.2度至0.5度的光斑，通过对置于水平或垂直轴上不同位置（以0.2度为步长）的单个光斑的皮层反应进行成像，来绘制成像视野的范围。通过移动呈现给每只眼睛的两个光斑的相对位置，可以将眼睛会聚评估到0.1度的分辨率范围内。一旦确定了合适的眼睛对齐方式，就可以放心地放置用于进一步光学成像程序的刺激物（例如用于研究视差反应的成像随机点刺激物）。此过程可以在整个实验中快速重复，以确保保持眼睛对齐。
